Photosensitizer-Loaded Multifunctional Chitosan Nanoparticles for Simultaneous in Situ Imaging, Highly Efficient Bacterial Biofilm Eradication, and Tumor Ablation.

Abstract

In recent decades, bacterial and viral infections and chronic inflammatory response have emerged as important causes of cancer. Also, infections remain a significant cause of morbidity and mortality in cancer patients. In this work, carboxymethyl chitosan nanoparticles (CMC NPs) were synthesized in a facile and green way and further combined with ammonium methylbenzene blue (MB) as a cross-linking agent as well as a fluorescent molecule and a photosensitizer for self-imaging photodynamic therapy (PDT). The obtained CMC-MB NPs exhibited an apparent pH-responsive release behavior of MB, which was released for a prolonged period in a simulated physiological environment (pH 7.4) for more than 15 days and the time reduced to only 3.5 h in acidic conditions (pH 5.5). When irradiated by a 650 nm laser at 202 mW/cm2 for 5 min, the CMC-MB NPs showed efficient bactericidal and biofilm eradication properties as well as suppression of tumor cell growth in a similar acidified microenvironment. Furthermore, in an in vivo rabbit wound bacterial infection model, the rapid sterilization of CMC-MB NPs played a crucial role in bacterial infections, inflammation inhibition, and wound healing. As a PDT treatment against cancer, the CMC-MB NPs also exhibited an efficient antitumor therapeutic effect in a subcutaneous tumor mice model.

• Authors: Lin Sun, Wenya Jiang, Hengrui Zhang, Yishun Guo, Wei Chen, Yingying Jin, Hao Chen, Kanghui Du, Hangdong Dai, Jian Ji, Bailiang Wang
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 11 Issue 2 Pg. 2302-2316 (Jan 16 2019) ISSN: 1944-8252 [Electronic] United States
• PMID: 30596498 (Publication Type: Journal Article)

Chemical References

• Photosensitizing Agents
• carboxymethyl-chitosan
• Chitosan

Topics

• Animals
• Bacteria (growth & development)
• Bacterial Infections (drug therapy, metabolism, pathology)
• Bacterial Physiological Phenomena (drug effects, radiation effects)
• Biofilms (drug effects, growth & development, radiation effects)
• Cell Line, Tumor
• Chitosan (analogs & derivatives, chemistry, pharmacology)
• Humans
• Mice
• Mice, Nude
• Nanoparticles (chemistry, therapeutic use)
• Neoplasms, Experimental (drug therapy, metabolism, pathology)
• Photochemotherapy
• Photosensitizing Agents (chemistry, pharmacology)
• Rabbits
• Xenograft Model Antitumor Assays