LncRNA SNHG20 contributes to cell proliferation and invasion by upregulating ZFX expression sponging miR-495-3p in gastric cancer.

Abstract

Long noncoding RNAs (lncRNAs) exert key regulators in cancer development and progression. The functional significance of lncRNA small nucleolar RNA host gene 20 (SNHG20) was reported in gastric cancer (GC); however, the underlying molecular mechanism in GC development is largely unknown. Here, our results showed that the lncRNA SNHG20 expression was significantly higher in GC tissues compared with adjacent normal tissues by quantitative real-time PCR (qRT-PCR) analysis. Higher lncRNA SNHG20 expression was highly associated with tumor size and lymphatic metastasis of patients. Patients with higher lncRNA SNHG20 expression predicted a short disease-free survival (DFS) and overall survival (OS). Furthermore, lncRNA SNHG20 expression negatively associated with miR-495-3p expression and regulated miR-495-3p expression. Function assays confirmed that lncRNA SNHG20 knockdown using RNA interference suppressed cell proliferation and invasion of GC by negatively regulating miR-495-3p expression. Moreover, we demonstrated that lncRNA SNHG20 inhibited zinc finger protein X-linked (ZFX) expression by negatively miR-495-3p expression in GC cells. In vivo, the current study also indicated that lncRNA SNHG20 knockdown reduced the tumor growth by downregulating ZFX expression. Thus, our results implied that inhibition of SNHG20/miR-495-3p/ZFX axis may provide valuable target for GC treatment.

• Authors: Ning Cui, Jun Liu, Hong Xia, Dong Xu
• Journal: Journal of cellular biochemistry (J Cell Biochem) Vol. 120 Issue 3 Pg. 3114-3123 (03 2019) ISSN: 1097-4644 [Electronic] United States
• PMID: 30520073 (Publication Type: Journal Article)
• Copyright: © 2018 Wiley Periodicals, Inc.

Chemical References

• Kruppel-Like Transcription Factors
• MIRN495 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• long non-coding RNA SNHG20, human
• zinc finger protein, X-linked

Topics

• Apoptosis (genetics, physiology)
• Blotting, Western
• Cell Line
• Cell Proliferation (genetics, physiology)
• Female
• Gene Expression Regulation, Neoplastic (genetics, physiology)
• Humans
• Kruppel-Like Transcription Factors (genetics, metabolism)
• Male
• MicroRNAs (genetics, metabolism)
• Middle Aged
• RNA, Long Noncoding (genetics, physiology)
• Stomach Neoplasms (genetics, metabolism, pathology)