The anti-inflammatory activity of peptides from simulated gastrointestinal digestion of preserved egg white in DSS-induced mouse colitis.

Abstract

Peptides DEDTQAMPFR (DR-10), MLGATSL (ML-7), SLSFASR (SR-7), and MSYSAGF (MF-7) derived from simulated gastrointestinal digestion of preserved egg white (SGD-PEW) exerted anti-inflammatory effects on Caco-2 cells. Here, we aimed to evaluate the anti-inflammatory effects of these peptides derived from SGD-PEW in a mouse model of dextran sodium sulfate (DSS)-induced colitis. The results showed that DR-10, ML-7, SR-7 and MF-7 significantly ameliorated the clinical symptoms of DSS-induced mice colitis, such as weight loss, disease activity index (DAI), colon shortening, spleen hypertrophy and histological scores. Treatment with DR-10, ML-7, SR-7 and MF-7 also significantly inhibited the local secretion of pro-inflammatory cytokines TNF-α and IL-6 and markedly decreased the gene expression of pro-inflammatory cytokines, including TNF-α, IL-6, IL-17, IL-1β, IFN-γ and MCP-1, in DSS-induced mice colitis. Overall, MF-7 showed the best effect of alleviating DSS-induced colitis among the four peptides. These results suggested that MF-7, DR-10, ML-7 and SR-7 may be a potential promising candidate for the treatment of inflammatory bowel disease (IBD).

• Authors: Mengya Zhang , Yan Zhao , Na Wu , Yao Yao , Mingsheng Xu , Huaying Du , Yonggang Tu
• Journal: Food & function (Food Funct) Vol. 9 Issue 12 Pg. 6444-6454 (Dec 13 2018) ISSN: 2042-650X [Electronic] England
• PMID: 30462121 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Interleukin-1beta
• Peptides
• Tumor Necrosis Factor-alpha
• Dextran Sulfate

Topics

• Animals
• Anti-Inflammatory Agents (chemistry, metabolism)
• Caco-2 Cells
• Cytokines (genetics, metabolism)
• Dextran Sulfate (adverse effects)
• Digestion
• Disease Models, Animal
• Ducks
• Egg White (chemistry)
• Female
• Humans
• Inflammatory Bowel Diseases (diet therapy, genetics, metabolism)
• Interleukin-1beta (genetics, metabolism)
• Mice
• Mice, Inbred BALB C
• Ovum (chemistry, metabolism)
• Peptides (chemistry, metabolism)
• Tumor Necrosis Factor-alpha (genetics, metabolism)