Abstract | BACKGROUND: METHODS: Female Wistar rats were randomly divided into three groups (n = 10): sham group, 5/6 nephrectomy (5/6 Nx) group, and lotensin group ( oral administration of lotensin for 9 weeks following 5/6 Nx). Rats were sacrificed and pathological parameters were measured. Western blot assay and immunohistochemical staining were performed to detect the expression of transforming growth factor-β1 (TGF-β1) and α-smooth muscle actin (α-SMA) in kidney tissues. RESULTS: Compared to the 5/6 Nx group, lotensin administration significantly decreased 5/6 Nx-induced elevation in blood urea nitrogen, serum creatinine and 24-h urinary protein excretion (UPE) rates, but markedly increased red blood cell count, plasma albumin and hemoglobin levels, along with improved renal morphology. Mechanistically, lotensin dramatically downregulated the renal expression of TGF-β1 and α-SMA induced by 5/6 Nx. CONCLUSIONS:
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Authors | Qingfei Xiao, Yinghui Guan, Chenhao Li, Li Liu, Dan Zhao, Hongyue Wang |
Journal | Renal failure
(Ren Fail)
Vol. 40
Issue 1
Pg. 583-589
(Nov 2018)
ISSN: 1525-6049 [Electronic] England |
PMID | 30371125
(Publication Type: Journal Article)
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Chemical References |
- Actins
- Benzazepines
- Transforming Growth Factor beta1
- smooth muscle actin, rat
- Creatinine
- benazepril
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Topics |
- Actins
(metabolism)
- Animals
- Benzazepines
(pharmacology)
- Blood Urea Nitrogen
- Creatinine
(blood)
- Female
- Immunohistochemistry
- Kidney
(drug effects, pathology)
- Kidney Failure, Chronic
(drug therapy, metabolism)
- Random Allocation
- Rats
- Rats, Wistar
- Transforming Growth Factor beta1
(metabolism)
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