Effects of irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA and induction of apoptosis in HeLa cells and nasal mucosa of rats.

Abstract

Narrowband-ultraviolet B (NB-UVB) phototherapy is used for the treatment of atopic dermatitis. Previously, we reported that irradiation with 200 mJ/cm2 of 310 nm NB-UVB suppressed phorbol-12-myristate-13-acetate (PMA)-induced up-regulation of histamine H1 receptor (H1R) gene expression without induction of apoptosis in HeLa cells. However, the effect of NB-UVB irradiation on nasal symptoms is still unclear. Here, we show that low dose irradiation with 310 nm NB-UVB alleviates nasal symptoms in toluene 2,4-diisocyanate (TDI)-sensitized allergy model rats. Irradiation with 310 nm NB-UVB suppressed PMA-induced H1R mRNA up-regulation in HeLa cells dose-dependently at doses of 75-200 mJ/cm2 and reversibly at a dose of 150 mJ/cm2 without induction of apoptosis. While, at doses of more than 200 mJ/cm2, irradiation with 310 nm NB-UVB induced apoptosis. Western blot analysis showed that the suppressive effect of NB-UVB irradiation on H1R gene expression was through the inhibition of ERK phosphorylation. In TDI-sensitized rat, intranasal irradiation with 310 nm NB-UVB at an estimated dose of 100 mJ/cm2 once a day for three days suppressed TDI-induced sneezes and up-regulation of H1R mRNA in nasal mucosa without induction of apoptosis. These findings suggest that repeated intranasal irradiation with low dose of NB-UVB could be clinically used as phototherapy of AR.

• Authors: Tatsuya Fujii, Yoshiaki Kitamura, Hiroyuki Mizuguchi, Kentaro Okamoto, Nanae Sanada, Takuya Yamada, Manabu Sugiyama, Shotaro Michinaga, Mika Kitayama, Hiroyuki Fukui, Noriaki Takeda
• Journal: Journal of pharmacological sciences (J Pharmacol Sci) Vol. 138 Issue 1 Pg. 54-62 (Sep 2018) ISSN: 1347-8648 [Electronic] Japan
• PMID: 30301597 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 The Authors. Production and hosting by Elsevier B.V. All rights reserved.

Chemical References

• RNA, Messenger
• Receptors, Histamine H1

Topics

• Animals
• Apoptosis (radiation effects)
• Dose-Response Relationship, Radiation
• Gene Expression (radiation effects)
• HeLa Cells
• Humans
• Male
• Nasal Mucosa (pathology, radiation effects)
• Phototherapy
• RNA, Messenger (metabolism)
• Rats
• Receptors, Histamine H1 (genetics, metabolism)
• Rhinitis, Allergic (therapy)
• Ultraviolet Rays
• Up-Regulation (radiation effects)