TREM1: A positive regulator for inflammatory response via NF-κB pathway in A549 cells infected with Mycoplasma pneumoniae.

Abstract

Herein, we found that serum content of the triggering receptor expressed on myeloid cells-1 (TREM1) was increased, and positively correlated with Mycoplasma pneumoniae (MP)-DNA in children with MP infection. In this study, A549 cells, known as human lung epithelial cells, were co-cultured with 107 CCU/ml of MP to established in vitro model of MP infection. We studied the roles of TREM1 in inflammatory response of A549 cell by regulating the secretions of cytokine interleukin (IL)-8 and tumor necrosis factor (TNF)-α in cell culture supernatants. Moreover, transcriptional activity of nuclear factor kappa B (NF-кB) was assessed by measuring protein levels of NF-кB in the cytoplasm and nuclear. Our data suggested that sanguinarine chloride significantly decreased TREM1 expression, and alleviated inflammatory response of MP-infected A549 cells via preventing NF-кB nuclear translocation. To study the roles of TREM1 in inflammatory regulation in MP-infected A549 cells and the underlying mechanisms, we established TREM1 overexpression transfected A549 cells. PDTC was used for inhibiting NF-кB nuclear translocation. We found that TREM1 overexpression induced server inflammatory response of A549 cells. Besides, TREM1 overexpression attenuated anti-inflammatory effects of sanguinarine chloride in MP-infected cells. More importantly, pro-inflammatory effects of TREM1 overexpression was significantly reversed with additional PDTC treatment in MP-infected cells treated with sanguinarine chloride, suggesting that TREM1 was a pro-inflammatory factor via regulating NF-кB nuclear translocation in MP-infected A549 cells.

• Authors: Fang Liu, XinGuang Zhang, Bin Zhang, WenWei Mao, TianTian Liu, Min Sun, YaHui Wu
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 107 Pg. 1466-1472 (Nov 2018) ISSN: 1950-6007 [Electronic] France
• PMID: 30257363 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Masson SAS. All rights reserved.

Chemical References

• Benzophenanthridines
• Interleukin-8
• Isoquinolines
• NF-kappa B
• TREM1 protein, human
• Triggering Receptor Expressed on Myeloid Cells-1
• Tumor Necrosis Factor-alpha
• sanguinarine

Topics

• A549 Cells
• Active Transport, Cell Nucleus
• Benzophenanthridines (pharmacology)
• Epithelial Cells (pathology)
• Gene Expression Regulation
• Humans
• Inflammation (genetics, microbiology, pathology)
• Interleukin-8 (metabolism)
• Isoquinolines (pharmacology)
• Lung (cytology, microbiology, pathology)
• Mycoplasma pneumoniae (isolation & purification)
• NF-kappa B (metabolism)
• Pneumonia, Mycoplasma (genetics, pathology)
• Triggering Receptor Expressed on Myeloid Cells-1 (blood, genetics)
• Tumor Necrosis Factor-alpha (metabolism)