Pancreatic-islet microvascular vasomotion dysfunction in mice with spinal cord injury.

Abstract

Patients with spinal cord injury (SCI) have an increased risk for developing type 2 diabetes. It is unknown whether the pancreatic-islet microvascular vasomotion is involved. We used female C57BL/6 mice and a 100-kilodyne T10 Infinite Horizons contusion SCI (or T10 laminectomy) to detect blood glucose and pancreatic-islet microvascular vasomotion. Blood glucose obtained from tail vein was detected using one Touch UltraEasy glucometer. Glucose tolerance test was performed by d-glucose administration intraperitoneally. Functional status of pancreatic-islet microvascular vasomotion was determined by laser Doppler monitoring. Expressions of insulin and glucagon were determined by immunohistochemistry. Expression of VEGF-A was determined by immunohistochemistry and Western blotting. Our result demonstrated that blood glucose was significantly increased at 4 h postinjury compared to that in sham group, with continuous higher blood glucose until 4 days postinjury (p < 0.05). SCI mice at day 7 and day 14 had significantly impaired glucose tolerance following glucose administration (p < 0.01). Average blood perfusion, amplitude, frequency, and relative velocity of vasomotion were significantly lower at 6 h postinjury than those in the sham group (p < 0.05), which were gradually upregulated over time. The expression of insulin was decreased, while the expression of glucagon was increased at 6 h postinjury. Similarly, the expression of VEGF-A was significantly decreased at 6 h postinjury, compared to that in sham group (p < 0.05), with slight increases by 14 days postinjury. Our study suggests that the functional status of pancreatic-islet microvascular vasomotion is impaired after injury, which may have implications for developing effective therapeutic interventions for SCI.

• Authors: Yingli Jing, Mingming Liu, Fan Bai, Di Li, Degang Yang
• Journal: Neuroscience letters (Neurosci Lett) Vol. 685 Pg. 68-74 (10 15 2018) ISSN: 1872-7972 [Electronic] Ireland
• PMID: 30125642 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018. Published by Elsevier B.V.

Chemical References

• Blood Glucose

Topics

• Animals
• Blood Glucose (metabolism)
• Diabetes Mellitus, Type 2 (metabolism, physiopathology)
• Disease Models, Animal
• Islets of Langerhans (metabolism, physiopathology)
• Mice, Inbred C57BL
• Microcirculation (physiology)
• Spinal Cord Injuries (physiopathology)