Sodium Butyrate Mitigates iE-DAP Induced Inflammation Caused by High-Concentrate Feeding in Liver of Dairy Goats.

Abstract

The aim of this study is to explore the impact of sodium butyrate on d-glutamyl- meso-diaminopimelic acid (iE-DAP)-induced liver inflammation in dairy goats during subacute ruminal acidosis (SARA) caused by high-concentrate feed. To achieve this aim, 12 lactating dairy goats were randomly divided into two groups: a high-concentrate feed group ( n = 6, concentrate/forage = 6:4) as the control group and a sodium butyrate (SB) with high-concentrate feed group ( n = 6, concentrate/forage = 6:4, with 1% SB by wt.) as the treatment group. A rumen pH below 5.6 lasted for at least 4 h/d due to long-term HC feeding. The concentration of iE-DAP was significantly lower (11.67 ± 3.85 μg/mL, and 7.74 ± 1.46 μg/mL, at the fourth h and sixth h of feeding, respectively) in the SB-treated group than that in the HC group (51.45 ± 5.71 μg/mL, and 18.31 ± 3.83 μg/mL, at the fourth h and sixth h of feeding, respectively). Meanwhile, SB significantly suppressed the mRNA expression of inflammatory genes (NOD1, RIPK2, TAK1, NF-κB/p65, ERK, JNK2, p38, IL-1β, TNF-α, CCL5, CCL20, CXCL12, FOS, β-defensin/LAP). Moreover, the protein expression of NOD1, p-IκBα, p-NF-κB/p-p65, p-ERK1/2, p-JNK, p-p38, and HDAC3 was significantly downregulated in the HC+SB group. In conclusion, iE-DAP-induced inflammation and liver disruption generated by the HC diet was mitigated by SB treatment.

• Authors: Animesh Chandra Roy, Yan Wang, Huanmin Zhang, Shipra Roy, Hongyu Dai, Guangjun Chang, Xiangzhen Shen
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 66 Issue 34 Pg. 8999-9009 (Aug 29 2018) ISSN: 1520-5118 [Electronic] United States
• PMID: 30078321 (Publication Type: Journal Article)

Chemical References

• Chemokine CCL20
• Cytokines
• Butyric Acid
• Diaminopimelic Acid
• N(2)-(gamma-D-glutamyl)-meso-2,2'-diaminopimelic acid

Topics

• Acidosis (drug therapy, immunology, metabolism, veterinary)
• Animal Feed (adverse effects, analysis)
• Animals
• Butyric Acid (administration & dosage)
• Chemokine CCL20 (genetics, immunology)
• Cytokines (genetics, immunology)
• Diaminopimelic Acid (adverse effects, analogs & derivatives)
• Goat Diseases (drug therapy, etiology, immunology, metabolism)
• Goats
• Liver (drug effects, immunology)
• Liver Diseases (drug therapy, etiology, immunology, veterinary)
• Rumen (drug effects, metabolism)