Golgi integral membrane protein 4 manipulates cellular proliferation, apoptosis, and cell cycle in human head and neck cancer.

Abstract

The incidence of global head and neck cancer has increased markedly in the last 10 years, and its prognosis is poor, which seriously endangers people's life and health. At present, there are few studies on its pathogenesis. Golgi integral membrane protein 4 (GOLIM4) is a major member of the Golgi apparatus transporter complex, and its role in tumor is unclear. The present study found that GOLIM4 was the key target protein downstream of stromal interaction molecule 1 (STIM1), which can inhibit the proliferation of head and neck cancer cells FaDu (human pharyngeal squamous carcinoma cell) and Tca-8113 (human tongue squamous carcinoma cell) with knockdown of GOLIM4 by lentivirus. And the decreased expression of GOLIM4 induced cellular apoptosis. Further experiments revealed that FaDu cell cycle progression was changed after GOLIM4 silence, G1 phase arrest and the number of G2/M cells decreased significantly. It was also found that the cells in S-phase decreased markedly after GOLIM4 was knocked down compared with the control group by 5-bromo-2'-deoxyuridine (BrdU) incorporation experiment. In conclusion, we found that GOLIM4, as the target gene downstream of STIM1, inhibited the proliferation of head and neck cancer, promoted apoptosis, and regulated cell cycle progression, and GOLIM4 is a novel oncogene in head and neck cancer and might help in developing promising targetted therapies for head and neck cancer patients.

• Authors: Yunfei Bai, Xiaobo Cui, Dongxue Gao, Yaping Wang, Boqian Wang, Wei Wang
• Journal: Bioscience reports (Biosci Rep) Vol. 38 Issue 4 (08 31 2018) ISSN: 1573-4935 [Electronic] England
• PMID: 30068697 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 The Author(s).

Chemical References

• GOLIM4 protein, human
• Vesicular Transport Proteins

Topics

• Apoptosis
• Cell Cycle
• Cell Line, Tumor
• Cell Proliferation
• Gene Expression Regulation, Neoplastic
• Gene Knockdown Techniques
• Head and Neck Neoplasms (genetics, metabolism, pathology)
• Humans
• Squamous Cell Carcinoma of Head and Neck (genetics, metabolism, pathology)
• Vesicular Transport Proteins (analysis, genetics, metabolism)