Srk1 kinase, a SR protein-specific kinase, is important for sexual reproduction, plant infection and pre-mRNA processing in Fusarium graminearum.

Abstract

SR protein-specific kinases (SRPKs) uniquely with a spacer region are important splicing factors from yeast to human. However, little is known about their biological functions in filamentous fungi. Therefore, we characterized a SRPK called SRK1 in wheat scab fungus Fusarium graminearum. Our data showed that Srk1 is required for vegetative growth, sexual reproduction and plant infection, and plays critical roles in pre-mRNA alternative splicing and gene expression. Remarkably, we found that Srk1 displayed dynamic shuttling between cytoplasm and the nucleus, which is regulated by the divergent spacer domain rather than its kinase activity, suggesting a regulatory mechanism for Srk1. Interestingly, Srk1-GFP also localized to the septal pores, indicating a possible role of Srk1 unrelated to mRNA processing. Although both K1 and K2 lobes of the kinase domain are essential for Srk1 functions, the K2 but not K1 lobe is responsible for the septal pore localization. Lastly, we established that Srk1 physically interacts with the two SR proteins, FgNpl3 and FgSrp1. Overall, our results indicated that SRK1 regulates fungal development, plant infection and mRNA processing by phosphorylation of other splicing factors including SR proteins, and the spacer domain regulates the functions of Srk1 by modulating its nucleocytoplasmic shuttling.

• Authors: Guanghui Wang, Peng Sun, Ziwen Gong, Lianfeng Gu, Yi Lou, Wenqin Fang, Lianhu Zhang, Li Su, Tao Yang, Baohua Wang, Jie Zhou, Jin-Rong Xu, Zonghua Wang, Wenhui Zheng
• Journal: Environmental microbiology (Environ Microbiol) Vol. 20 Issue 9 Pg. 3261-3277 (09 2018) ISSN: 1462-2920 [Electronic] England
• PMID: 30051568 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.

Chemical References

• Fungal Proteins
• RNA Precursors
• Protein Kinases

Topics

• Cell Nucleus (enzymology, genetics)
• Fungal Proteins (genetics, metabolism)
• Fusarium (enzymology, genetics, pathogenicity, physiology)
• Humans
• Phosphorylation
• Plant Diseases (microbiology)
• Protein Binding
• Protein Kinases (genetics, metabolism)
• RNA Precursors (genetics, metabolism)
• RNA Processing, Post-Transcriptional
• Saccharomyces cerevisiae (genetics)
• Spores, Fungal (enzymology, genetics, growth & development)
• Triticum (microbiology)
• Virulence