Hsa-mir-127 impairs survival of patients with glioma and promotes proliferation, migration and invasion of cancerous cells by modulating replication initiator 1.

Abstract

This work aimed to investigate the inter-regulatory functions of hsa-mir-127 and replication initiator 1 (REPIN1) on the proliferation and metastasis of glioma cells. The in-silico data on the implication of hsa-mir-127 and REPIN1 in glioma were retrieved from The Cancer Genome Atlas (TCGA). The expression levels of hsa-mir-127 and REPIN1 mRNA were determined by qRT-PCR, whereas Western blot was used to detect REPIN1 protein expression in glioma cell lines. The proliferation of glioma cells was determined by means of the MTT assay, whereas the transwell assay was employed for assessing the extent of cell migration and invasion. The interaction among REPIN1 and hsa-mir-127 was checked using the luciferase reporter assay. The expression of hsa-mir-127 was markedly increased in clinical data obtained from TCGA and in glioma cells compared with normal tissues and control cells, respectively. Increased expression of hsa-mir-127 and decreased expression of REPIN1 were both associated with poor overall survival. Moreover, hsa-mir-127 overexpression noticeably promoted proliferation, inhibited apoptosis and increased the invasive and migratory capacities of glioma cells. Inverse effects were found with hsa-mir-127 antisense inhibitor. Interestingly, overexpression of hsa-mir-127 downregulated REPIN1 expression, and luciferase reporter assay showed that the tumorigenesis effect of hsa-mir-127 requires, in part, its direct targeting of REPIN1. In conclusion, the hsa-mir-127/REPIN1 pathway is involved in gliomas and could be a potential therapeutic target.

• Authors: Yunling Wang, Yihai Lin
• Journal: Neuroreport (Neuroreport) Vol. 29 Issue 14 Pg. 1166-1173 (09 26 2018) ISSN: 1473-558X [Electronic] England
• PMID: 29979259 (Publication Type: Journal Article)

Chemical References

• Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
• DNA-Binding Proteins
• MIRN127 microRNA, human
• MicroRNAs
• REPIN1 protein, human
• RNA-Binding Proteins

Topics

• Basic Helix-Loop-Helix Leucine Zipper Transcription Factors (genetics)
• Brain Neoplasms (genetics, metabolism)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• DNA-Binding Proteins
• Down-Regulation
• Gene Expression Regulation, Neoplastic (genetics)
• Glioma (genetics, metabolism)
• Humans
• MicroRNAs (genetics)
• RNA-Binding Proteins