Identification of recurrence-associated microRNAs in stage I lung adenocarcinoma.

Abstract

Lung cancer is the most common cause of cancer-associated death worldwide. Postoperative relapse and subsequent metastasis result in a high mortality rate, even in early stage lung cancer. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level and are frequently dysregulated in various cancers. The aim of this study was to identify recurrence-associated miRNAs in early stage lung cancer. To screen for differentially expressed miRNAs related to postoperative recurrence, miRNA microarray data derived from stage I lung adenocarcinoma formalin-fixed paraffin-embedded (FFPE) tissue samples (n = 6) and publically available the Cancer Genome Atlas (TCGA) data were analyzed. An independent sample (n = 29) was used to validate candidate miRNAs by quantitative real-time polymerase chain reaction (qRT-PCR). In miRNA expression profiling, we identified 60 significantly dysregulated miRNAs in the relapsed group. Additionally, 20 dysregulated miRNAs were found using TCGA data set. Three miRNAs (let-7g-5p, miR-143-3p, and miR-374a-5p) were associated with postoperative recurrence in both microarray and TCGA data sets. All 3 candidate miRNAs were validated in the independent cohort of stage I adenocarcinoma by qRT-PCR. We discovered 3 recurrence-associated miRNAs of stage I lung adenocarcinoma samples using FFPE tissue, which showed possible clinical utility as biomarkers predicting recurrence after curative surgery. Further investigation of the functional properties of these miRNAs is needed.

• Authors: Jongmin Sim, Yeseul Kim, Hyunsung Kim, Su-Jin Shin, Dong-Hoon Kim, Seung Sam Paik, Kiseok Jang
• Journal: Medicine (Medicine (Baltimore)) Vol. 97 Issue 25 Pg. e10996 (Jun 2018) ISSN: 1536-5964 [Electronic] United States
• PMID: 29923982 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• MIRN143 microRNA, human
• MicroRNAs

Topics

• Adenocarcinoma (genetics, pathology, surgery)
• Adenocarcinoma of Lung
• Adult
• Aged
• Biomarkers, Tumor (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms (genetics, pathology, surgery)
• Male
• MicroRNAs (genetics)
• Middle Aged
• Neoplasm Recurrence, Local (genetics, pathology)
• Neoplasm Staging
• Pneumonectomy (adverse effects, methods)
• Republic of Korea