Abstract | BACKGROUND & OBJECTIVES: METHODS: A total of 145 patients including those with fever and lymphadenopathy, infective endocarditis and neuroretinitis were enrolled in the study. Whole blood, serum and lymph node aspirate and valvular vegetations if available, were obtained. Samples were plated on chocolate agar and brain-heart infusion agar containing five per cent fresh rabbit blood and were incubated at 35°C for at least four weeks in five per cent CO2with high humidity. Immunofluorescent antibody assay (IFA) was done for the detection of IgM antibodies in the serum using a commercial kit. Whole blood was used to perform polymerase chain reaction (PCR) for the citrate synthase gene (gltA). RESULTS: IFA was positive in 11 of 140 (7.85%) patients and PCR was positive in 3 of 140 (2.14%) patients. Culture was negative in all the cases. A higher incidence of Bartonella infection was seen in patients with fever and lymphadenopathy ( n=30), seven of whom were children. In ophthalmological conditions, four cases were IFA positive. INTERPRETATION & CONCLUSIONS: The present study shows that the threat of Bartonella infection is a reality in India. It is also an important treatable cause of fever and lymphadenopathy in children. Serology and PCR are useful tests for its diagnosis. Clinicians should consider. BARTONELLA:
|
Authors | Rama Chaudhry, Prathyusha Kokkayil, Arnab Ghosh, Tej Bahadur, Kamala Kant, Tanu Sagar, Sunil Kumar Kabra, Rakesh Lodha, Aparajit Ballav Dey, Vimala Menon |
Journal | The Indian journal of medical research
(Indian J Med Res)
Vol. 147
Issue 2
Pg. 189-194
(Feb 2018)
ISSN: 0971-5916 [Print] India |
PMID | 29806608
(Publication Type: Journal Article)
|
Chemical References |
- Antibodies, Bacterial
- Citrate (si)-Synthase
|
Topics |
- Adolescent
- Adult
- Animals
- Antibodies, Bacterial
(blood)
- Bartonella Infections
(blood, microbiology, transmission)
- Bartonella henselae
(isolation & purification, pathogenicity)
- Cat-Scratch Disease
(epidemiology, transmission)
- Cats
- Child
- Citrate (si)-Synthase
(blood)
- Disease Reservoirs
- Female
- Humans
- India
(epidemiology)
- Lymphadenopathy
(blood, microbiology, pathology)
- Male
- Middle Aged
- Rabbits
- Rats
- Tertiary Care Centers
- Young Adult
- Zoonoses
(blood, epidemiology, microbiology, pathology)
|