Inhibition of VEGF gene expression in osteoblast cells by different NSAIDs.

Abstract

OBJECTIVE:

To determine the effect of different nonsteroidal anti-inflammatory drugs (NSAIDs) on vascular endothelial growth factor (VEGF) gene expression in two osteoblast cell populations.

DESIGN:

Osteoblasts obtained by primary culture (HOp) and human osteosarcoma cell line MG63 (MG-63), which were treated with 10 μM doses of acetaminophen, indomethacin, ketoprofen, diclofenac, ibuprofen, ketorolac, naproxen or piroxicam. At 24 h of treatment, their gene expression of VEGF was evaluated by real-time polymerase chain reaction (RT-PCR) and compared with the expression in untreated cells (control group).

RESULTS:

The treatment with the different NSAIDs significantly reduced VEGF expression regardless of the cell line and NSAID studied.

CONCLUSION:

The results of this study suggest that these drugs may have undesirable effects on the osteoblast and its bone-forming capacity, given the effect of this growth factor on these cells. Further studies are warranted to determine their repercussions on bone tissue and to elucidate the cell signaling mechanism/s involved.

• Authors: Francisco Javier Manzano-Moreno, Victor Javier Costela-Ruiz, Lucía Melguizo-Rodríguez, Rebeca Illescas-Montes, Olga García-Martínez, Concepción Ruiz, Javier Ramos-Torrecillas
• Journal: Archives of oral biology (Arch Oral Biol) Vol. 92 Pg. 75-78 (Aug 2018) ISSN: 1879-1506 [Electronic] England
• PMID: 29763781 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Vascular Endothelial Growth Factor A

Topics

• Adult
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Cell Line
• Cells, Cultured
• Female
• Gene Expression
• Humans
• Male
• Osteoblasts (drug effects, metabolism)
• Real-Time Polymerase Chain Reaction
• Vascular Endothelial Growth Factor A (metabolism)