Abstract | OBJECTIVE: DESIGN: Immunophenotyping, immunohistochemistry, T cell receptor profiling and RNA sequencing were performed using blood and colonic biopsies from patients with IBD before and during treatment with vedolizumab (n=18) or, as control, the anti-TNFα antibody infliximab (n=20). Leucocyte trafficking in vivo was assessed using single photon emission computed tomography and endomicroscopy. RESULTS:
Vedolizumab was not associated with alterations in the abundance or phenotype of lamina propria T cells and did not affect the mucosal T cell repertoire or leucocyte trafficking in vivo. Surprisingly, however, α4β7 antibody treatment was associated with substantial effects on innate immunity including changes in macrophage populations and pronounced alterations in the expression of molecules involved in microbial sensing, chemoattraction and regulation of the innate effector response. These effects were specific to vedolizumab, not observed in response to the TNFα antibody infliximab, and associated with inhibition of intestinal inflammation. CONCLUSION: Our findings suggest that modulation of innate immunity contributes to the therapeutic efficacy of vedolizumab in IBD. TRIAL REGISTRATION NUMBER: NCT02694588.
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Authors | Sebastian Zeissig, Elisa Rosati, C Marie Dowds, Konrad Aden, Johannes Bethge, Berenice Schulte, Wei Hung Pan, Neha Mishra, Maaz Zuhayra, Marlies Marx, Maren Paulsen, Anne Strigli, Claudio Conrad, Dörthe Schuldt, Anupam Sinha, Henriette Ebsen, Sabin-Christin Kornell, Susanna Nikolaus, Alexander Arlt, Dieter Kabelitz, Mark Ellrichmann, Ulf Lützen, Philip C Rosenstiel, Andre Franke, Stefan Schreiber |
Journal | Gut
(Gut)
Vol. 68
Issue 1
Pg. 25-39
(01 2019)
ISSN: 1468-3288 [Electronic] England |
PMID | 29730603
(Publication Type: Journal Article, Observational Study, Research Support, Non-U.S. Gov't)
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Copyright | © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2019. All rights reserved. No commercial use is permitted unless otherwise expressly granted. |
Chemical References |
- Antibodies, Monoclonal, Humanized
- Gastrointestinal Agents
- Integrins
- integrin alpha4beta7
- vedolizumab
- Infliximab
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Topics |
- Adaptive Immunity
(drug effects)
- Adult
- Antibodies, Monoclonal, Humanized
(therapeutic use)
- Biopsy
- Case-Control Studies
- Female
- Gastrointestinal Agents
(therapeutic use)
- Humans
- Immunity, Innate
(drug effects)
- Immunohistochemistry
- Immunophenotyping
- Inflammatory Bowel Diseases
(drug therapy, immunology)
- Infliximab
(therapeutic use)
- Integrins
(antagonists & inhibitors)
- Male
- Phenotype
- Prospective Studies
- Sequence Analysis, RNA
- T-Lymphocytes
(immunology)
- Tomography, Emission-Computed, Single-Photon
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