Abstract |
The present study investigates the impact of germinated brown rice (GBR) on atherosclerosis and the underlying mechanism in low-density lipoprotein receptor-knockout (LDLr-KO) mice. The intensity of atherosclerosis in aortas of LDLr-KO mice receiving diet supplemented with 60% GBR (weight/weight) was significantly less than that in mice fed with 60% white rice (WR) or control diet ( p < 0.05); all diets contained 0.06% cholesterol. WR or GBR diet did not significantly alter plasma total or LDL-cholesterol, fecal sterols, or glucose, or the activities of antioxidant enzymes, compared to the control diet. The adhesion of monocytes to aortas from LDLr-KO mice fed with WR diet was significantly more than that from mice receiving the control diet ( p < 0.01). GBR diet decreased monocyte adhesion to aortas compared to WR diet ( p < 0.01). GBR diet also reduced the levels of plasminogen activator inhibitor-1 (PAI-1), monocyte chemotactic protein-1 (MCP-1), and tumor necrosis factor-α (TNF-α) in plasma, and the abundances of MCP-1, PAI-1, TNF-α, intracellular cell adhesion molecule-1, toll-like receptor-4, PAI-1, LDLr-like protein, and urokinase plasminogen activator and its receptor in aortas or hearts from LDLr-KO mice in comparison to the WR diet ( p < 0.05, 0.01, respectively). The findings suggest that GBR administration attenuated atherosclerosis and vascular inflammation in LDLr-KO mice compared to WR. The anti-atherosclerotic effect of GBR in LDLr-KO mice at least in part results from its anti-inflammatory activity.
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Authors | Ruozhi Zhao, Nora Ghazzawi, Jiansu Wu, Khuong Le, Chunyang Li, Mohammed H Moghadasian, Yaw L Siow, Franklin B Apea-Bah, Trust Beta, Zhengfeng Yin, Garry X Shen |
Journal | Journal of agricultural and food chemistry
(J Agric Food Chem)
Vol. 66
Issue 17
Pg. 4512-4520
(May 02 2018)
ISSN: 1520-5118 [Electronic] United States |
PMID | 29656648
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents
- Ccl2 protein, mouse
- Chemokine CCL2
- Lipids
- Plasminogen Activator Inhibitor 1
- Receptors, LDL
- Tumor Necrosis Factor-alpha
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Topics |
- Animals
- Anti-Inflammatory Agents
- Atherosclerosis
(diet therapy, prevention & control)
- Chemokine CCL2
(blood)
- Diet
- Germination
- Lipids
(blood)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Oryza
- Plasminogen Activator Inhibitor 1
(blood)
- Receptors, LDL
(deficiency, genetics, physiology)
- Tumor Necrosis Factor-alpha
(blood)
- Vasculitis
(prevention & control)
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