Absences of Endothelial Microvesicle Changes in the Presence of the Endotheliopathy of Trauma.

Abstract

INTRODUCTION:

Severe trauma is accompanied by endothelial glycocalyx disruption, which drives coagulopathy, increasing transfusion requirements and death. This syndrome has been termed endotheliopathy of trauma (EOT). Some have suggested EOT results from endothelial cellular damage and apoptosis. Endothelial microvesicles (EMVs) represent cellular damage. We hypothesized that EOT is associated with endothelial damage and apoptosis resulting in an increase in circulating EMVs.

METHODS:

Prospective, observational study enrolling severely injured patients. Twelve patients with EOT, based on elevated Syndecan-1 levels, were matched with 12 patients with lower levels, based on Injury Severity Score (ISS), abbreviated injury scale profile, and age. Thrombelastography and plasma levels of biomarkers indicative of cellular damage were measured from blood samples collected on admission. EMVs were determined by flow cytometry using varied monoclonal antibodies associated with endothelial cells. Significance was set at P < 0.05.

RESULTS:

Admission physiology and ISS (29 vs. 28) were similar between groups. Patients with EOT had higher Syndecan-1, 230 (158, 293) vs. 19 (14, 25) ng/mL, epinephrine, and soluble thrombomodulin levels. Based on thrombelastography, EOT had reductions in clot initiation, amplification, propagation and strength, and a greater frequency of transfusion, 92% vs. 33%. There were no differences in EMVs irrespective of the antibody used. Plasma norepinephrine, sE-selectin, sVE-cadherin, and histone-complexed DNA fragments levels were similar.

CONCLUSION:

In trauma patients presenting with EOT, endothelial cellular damage or apoptosis does not seem to occur based on the absence of an increase in EMVs and other biomarkers. Thus, this suggests endothelial glycocalyx disruption is the underlying primary cause of EOT.

• Authors: Charles E Wade, Nena Matijevic, Yao-Wei W Wang, Erika G Rodriguez, Ernesto Lopez, Sisse R Ostrowski, Jessica C Cardenas, Lisa A Baer, Tzu-An Chen, Jeffrey S Tomasek, Hanne H Henriksen, Jakob Stensballe, Bryan A Cotton, John B Holcomb, Pär I Johansson
• Journal: Shock (Augusta, Ga.) (Shock) Vol. 51 Issue 2 Pg. 180-184 (02 2019) ISSN: 1540-0514 [Electronic] United States
• PMID: 29621120 (Publication Type: Clinical Trial, Journal Article, Observational Study)

Chemical References

• Biomarkers

Topics

• Adult
• Apoptosis
• Biomarkers (blood)
• Blood Coagulation Disorders (blood, etiology, pathology)
• Endothelial Cells (metabolism, pathology)
• Female
• Glycocalyx (metabolism, pathology)
• Humans
• Injury Severity Score
• Male
• Middle Aged
• Wounds and Injuries (blood, complications, pathology)