Abstract | BACKGROUND: MATERIALS AND METHODS: Quantitative reverse transcription polymerase chain reaction, wound healing, transwell migration and invasion assays, Western blotting, and xenograft tumor growth models were performed to test the expression levels and functions of miR-212 in hepatocellular carcinoma. Luciferase reporter assay, quantitative reverse transcription polymerase chain reaction, Western blotting, and immunohistochemistry were used to identify and verify the target of miR-212. RESULTS: In this study, we identify significant repression of miR-212 in hepatocellular carcinoma and demonstrate that overexpression of miR-212 inhibits the migration of hepatocellular carcinoma cells in vitro and in vivo. Furthermore, we identify forkhead box M1, whose expression is inversely related to that of miR-212, as a direct target of miR-212. Additionally, reexpression of forkhead box M1 rescues the miR-212-mediated inhibition of cell migration. We observed that inhibition of miR-212 activates forkhead box M1 but inhibits the Wnt/β- catenin pathway by suppressing Wnt, LEF-1, c-Myc, and nuclear β- catenin. Finally, in vivo studies confirmed the inhibitory effect of miR-212 on hepatocellular carcinoma growth. CONCLUSION:
|
Authors | Pengbo Jia, Guangbing Wei, Cancan Zhou, Qi Gao, Yunhua Wu, Xuejun Sun, Xuqi Li |
Journal | Technology in cancer research & treatment
(Technol Cancer Res Treat)
Vol. 17
Pg. 1533034618765221
(01 01 2018)
ISSN: 1533-0338 [Electronic] United States |
PMID | 29618288
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- 3' Untranslated Regions
- MIRN212 microRNA, human
- MicroRNAs
|
Topics |
- 3' Untranslated Regions
- Animals
- Carcinoma, Hepatocellular
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Cell Transformation, Neoplastic
(genetics, metabolism)
- Disease Models, Animal
- Gene Expression Regulation, Neoplastic
- Genes, Reporter
- Humans
- Liver Neoplasms
(genetics, metabolism, pathology)
- Mice
- MicroRNAs
(genetics)
- RNA Interference
- Wnt Signaling Pathway
- Xenograft Model Antitumor Assays
|