A Stool Multitarget mRNA Assay for the Detection of Colorectal Neoplasms.

Abstract

Noninvasive screening methods for the detection of colorectal cancers (CRC) at curable stages rely on the identification of specific biomarkers. Our group has shown that mRNA stool assays represent a powerful and robust approach for the prediction of colorectal neoplasms. In this methodological chapter, we describe the procedures to isolate good quality stool RNA and the steps to evaluate the levels of specific host mRNA markers such as ITGA6, MYC, and GADD45B using TaqMan-based quantitative and droplet digital PCR approaches.

• Authors: Elizabeth Herring, Shigeru Kanaoka, Eric Tremblay, Jean-François Beaulieu
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 1765 Pg. 217-227 ( 2018) ISSN: 1940-6029 [Electronic] United States
• PMID: 29589311 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Differentiation
• Biomarkers, Tumor
• GADD45B protein, human
• ITGA6 protein, human
• Integrin alpha6
• MYC protein, human
• Proto-Oncogene Proteins c-myc
• RNA, Messenger

Topics

• Antigens, Differentiation (genetics)
• Biomarkers, Tumor (analysis)
• Colorectal Neoplasms (diagnosis)
• Early Detection of Cancer (instrumentation, methods)
• Feces (chemistry)
• Humans
• Integrin alpha6 (genetics)
• Proto-Oncogene Proteins c-myc (genetics)
• RNA, Messenger (analysis)
• Real-Time Polymerase Chain Reaction (instrumentation, methods)