Identification of Response Elements on Promoters Using Site-Directed Mutagenesis and Chromatin Immunoprecipitation.

Abstract

Proximal promoters are located upstream of the transcription start sites of genes, and they contain regulatory sequences on which bind different transcription factors for promoting colorectal cancer progression. Here we describe the comprehensive methodology used previously for the identification and functional characterization of MYC-responsive elements in the integrin α1 subunit (ITGA1) gene using a combination of in silico analysis, site-directed mutagenesis, and chromatin immunoprecipitation.

• Authors: Salah Boudjadi, Julie C Carrier, Jean-François Beaulieu
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 1765 Pg. 43-56 ( 2018) ISSN: 1940-6029 [Electronic] United States
• PMID: 29589300 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Integrin alpha1
• MYC protein, human
• Proto-Oncogene Proteins c-myc

Topics

• Binding Sites
• Carcinogenesis (genetics)
• Chromatin Immunoprecipitation (instrumentation, methods)
• Colorectal Neoplasms (genetics)
• Computer Simulation
• Gene Expression Regulation, Neoplastic
• HEK293 Cells
• Humans
• Integrin alpha1 (genetics)
• Mutagenesis, Site-Directed (instrumentation, methods)
• Proto-Oncogene Proteins c-myc (metabolism)
• Response Elements (genetics)
• Sequence Analysis, DNA (methods)
• Transcription Initiation Site