Combined Extracts of Artemisia and Green Tea, Mitigated Alcoholic Gastritis Via Enhanced Heat-shock Protein 27.

Abstract

Background/Aims:

Several lines of evidence from epidemiologic and laboratory studies have shown that the consumption of Artemisia or green tea extracts (MPGT) is inversely associated with the risk of alcohol-induced damage and other chronic diseases. Supported by previous studies showing that the combined extract of Artemisia and green tea, MPGT, exerted significantly either antioxidative or anti-inflammatory actions against Helicobacter pylori-associated gastric diseases, it was hypothesized that MPGT can offer protection against alcoholic gastritis.

Methods:

Ethanol was administered to induce gastric damage in Wistar rats, which had been pretreated with various doses of MPGT, to measure the rescuing action of a MPGT pretreatment against ethanol-induced gastric damage. In addition, the molecular mechanisms for the preventive effects were examined.

Results:

The MPGT pretreatment (100, 300, and 500 mg/kg) alleviated the ethanol-induced gastric damage, which was evidenced by the significant decrease in calcium-dependent phospholipase A2, MAPKs, and NF-κB levels compared to ethanol alone. Furthermore, the MPGT pretreatment preserved 15-prostaglandin dehydrogenase, whereas cyclooxygenase-2 was decreased significantly. All of these biochemical changes led to the significant alleviation of alcohol-associated gastric mucosal damage. Ethanol significantly increased the TUNEL positivity in the stomach, but MPGT decreased the apoptotic index significantly, which was associated with significantly lower pathological scores of ethanol-induced mucosal ulcerations. The significant protective changes observed alcoholic gastritis with MPGT were related to the increased expression of cytoprotective genes, such as heat-shock protein (HSP)27, HSP60, and PDGF.

Conclusions:

The efficient anti-inflammatory, anti-apoptotic, and regenerative actions of MPGT make it a potential nutrient phytoceutical to rescue the stomach from alcoholic gastritis.

• Authors: Yong Seok Kim, Migyeong Jeong, Young Min Han, Jong Min Park, Sang Oh Kwon, Seong Pyo Hong, Ki Baik Hahm
• Journal: The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi (Korean J Gastroenterol) Vol. 71 Issue 3 Pg. 132-142 (03 25 2018) ISSN: 2233-6869 [Electronic] Korea (South)
• PMID: 29566474 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HSP27 Heat-Shock Proteins
• NF-kappa B
• Plant Extracts
• Platelet-Derived Growth Factor
• Tea
• Ethanol
• Cyclooxygenase 2
• Phospholipases A2

Topics

• Animals
• Artemisia (chemistry, metabolism)
• Cyclooxygenase 2 (metabolism)
• Ethanol (toxicity)
• Gastric Mucosa (pathology)
• Gastritis (chemically induced, pathology, prevention & control, veterinary)
• HSP27 Heat-Shock Proteins (genetics, metabolism)
• Male
• NF-kappa B (metabolism)
• Phospholipases A2 (metabolism)
• Plant Extracts (chemistry, pharmacology, therapeutic use)
• Platelet-Derived Growth Factor (genetics, metabolism)
• Rats
• Rats, Wistar
• Tea (chemistry, metabolism)
• Up-Regulation (drug effects)