Abstract | BACKGROUND: METHODS: We used the quantitative real-time PCR technique to analyze differences in the expression levels of the ESR1 and GPX1 genes in paired malignant and non-malignant tissues from breast cancer patients. RESULTS: ESR1 and GPX1 expression levels were found to be significantly down-regulated by 14.7% and 7.4% (respectively) in the tumorous breast tissue when compared to the non-malignant one. Down-regulation of these genes was independent of the tumor histopathology classification and clinicopathological factors, while the ESR1 mRNA level was reduced with increasing tumor grade (G1: 103% vs. G2: 85.8% vs. G3: 84.5%; p<0.05). In the non-malignant and malignant breast tissues, the expression levels of ESR1 and GPX1 were significantly correlated with each other (Rs=0.450 and Rs=0.360; respectively). CONCLUSION: Our data suggest that down-regulation of ESR1 and GPX1 was independent of clinicopathological factors. Down-regulation of ESR1 gene expression was enhanced by the development of the disease. Moreover, GPX1 and ESR1 gene expression was interdependent in the malignant breast tissue and further work is needed to determine the mechanism underlying this relationship.
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Authors | Magdalena B Król, Michał Galicki, Peter Grešner, Edyta Wieczorek, Ewa Jabłońska, Edyta Reszka, Zbigniew Morawiec, Wojciech Wąsowicz, Jolanta Gromadzińska |
Journal | Acta biochimica Polonica
(Acta Biochim Pol)
Vol. 65
Issue 1
Pg. 51-57
( 2018)
ISSN: 1734-154X [Electronic] Poland |
PMID | 29543921
(Publication Type: Journal Article)
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Chemical References |
- ESR1 protein, human
- Estrogen Receptor alpha
- RNA, Messenger
- Glutathione Peroxidase
- Glutathione Peroxidase GPX1
- GPX1 protein, human
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Topics |
- Breast
(cytology, metabolism)
- Breast Neoplasms
(metabolism, pathology)
- Down-Regulation
- Estrogen Receptor alpha
(genetics, metabolism)
- Female
- Gene Expression Regulation, Neoplastic
- Glutathione Peroxidase
(genetics, metabolism)
- Humans
- Polymerase Chain Reaction
- RNA, Messenger
(analysis)
- Glutathione Peroxidase GPX1
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