Abstract | BACKGROUND/AIMS: METHODS: Real-time quantitative PCR was used to determine the expression level of SNHG12 in cervical cancer tissues and cell lines. Loss-of-function assays were performed to examine the effect of SNHG12 on the proliferation, apoptosis, migration and invasion of cervical cancer cells in vitro and tumor growth in vivo. Luciferase experiments were employed to explore the interactions between SNHG12 and miR-424-5p. RESULTS: SNHG12 was found to be abnormally elevated in human cervical cancer tissues compared with paired adjacent normal tissues. Moreover, high SNHG12 expression in tumor tissues was significantly correlated with vascular involvement, lymph node metastasis, advanced FIGO stage and poor prognosis. Furthermore, the knockdown of SNHG12 was found to inhibit proliferation, migration and invasion of cervical cancer cells in vitro, and silencing SNHG12 was shown to suppress tumor growth in a nude mouse model. Mechanistic studies showed that SNHG12 functioned as an endogenous sponge for miR-424-5p, thereby downregulating the expression of miR-424-5p in cervical cancer. Furthermore, the inhibition of miR-424-5p in SNHG12-depleted cells partially reversed the effects on cervical cancer cell apoptosis, adhesion and invasion. CONCLUSION: In summary, our findings suggest that the tumor-promoting role of SNHG12 is to function as a molecular sponge, which negatively regulates miR-424-5p. These findings may provide a potent therapeutic target for cervical cancer.
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Authors | Jing Dong, Qing Wang, Li Li, Zhang Xiao-Jin |
Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
(Cell Physiol Biochem)
Vol. 45
Issue 5
Pg. 2086-2094
( 2018)
ISSN: 1421-9778 [Electronic] Germany |
PMID | 29533945
(Publication Type: Journal Article)
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Copyright | © 2018 The Author(s). Published by S. Karger AG, Basel. |
Chemical References |
- 3' Untranslated Regions
- MIRN424 microrna, human
- MicroRNAs
- RNA, Long Noncoding
- SNHG12 long non-coding RNA, human
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Topics |
- 3' Untranslated Regions
- Adult
- Animals
- Apoptosis
- Base Sequence
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Disease Progression
- Down-Regulation
- Female
- HeLa Cells
- Humans
- Mice
- Mice, Nude
- MicroRNAs
(genetics, metabolism)
- Middle Aged
- Neoplasm Staging
- Prognosis
- RNA, Long Noncoding
(antagonists & inhibitors, genetics, metabolism)
- Sequence Alignment
- Transplantation, Heterologous
- Uterine Cervical Neoplasms
(genetics, pathology)
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