In this study, we investigated the mechanism by which
lncRNA metastasis-associated
lung adenocarcinoma transcript 1 (MALAT1) mediates
cisplatin resistance in
lung cancer.
Lung cancer patients with high MALAT1 levels were associated with
cisplatin resistance and low overall survival. Moreover,
cisplatin-resistant A549/DDP cells showed higher MALAT1 expression than
cisplatin-sensitive
lung cancer cells (A549, H460, H1299 and SPC-A1). Dual
luciferase reporter and
RNA immunoprecipitation assays showed direct binding of miR-101-3p to MALAT1. MALAT1 knockdown in
lung cancer cells resulted in miR-101-3p upregulation and increased
cisplatin sensitivity. In addition, miR-101-3p decreased myeloid cell
leukemia 1 (MCL1) expression by binding to the 3'-untranslated region (3'-UTR) of its
mRNA. These results demonstrate that MALAT1/miR-101-3p/MCL1 signaling underlies
cisplatin resistance in
lung cancer.