Leucocyte and Platelet-rich Fibrin: a carrier of autologous multipotent cells for regenerative medicine.

Abstract

The wound healing is a complex process wherein inflammation, proliferation and regeneration evolve according to a spatio-temporal pattern from the activation of coagulation cascade to the formation of a plug clot including fibrin matrix, blood-borne cells and cytokines/growth factors. Creating environments conducive to tissue repair, the haemoderivatives are commonly proposed for the treatment of hard-to-heal wounds. Here, we explored in vitro the intrinsic regenerative potentialities of a leucocyte- and platelet-rich fibrin product, known as CPL-MB, defining the stemness grade of cells sprouting from the haemoderivative. Using highly concentrated serum-based medium to simulate wound conditions, we isolated fibroblast-like cells (CPL-CMCs) adhering to plastic and showing stable in vitro propagation, heterogeneous stem cell expression pattern, endothelial adhesive properties and immunomodulatory profile. Due to their blood derivation and expression of CXCR4, CPL-CMCs have been suggested to be immature cells circulating in peripheral blood at quiescent state until activation by both coagulation event and inflammatory stimuli such as stromal-derived factor 1/SDF1. Expressing integrins (CD49f, CD103), vascular adhesion molecules (CD106, CD166), endoglin (CD105) and remodelling matrix enzymes (MMP2, MMP9, MMP13), they showed a transendothelial migratory potential besides multipotency. Taken together, our data suggested that a standardized, reliable and economically feasible blood product such as CPL-MB functions as an artificial stem cell niche that, under permissive conditions, originate ex vivo immature cells that could be useful for autologous stem cell-based therapies.

• Authors: Rosa Di Liddo, Thomas Bertalot, Alessio Borean, Ivan Pirola, Alberto Argentoni, Sandra Schrenk, Carola Cenzi, Stefano Capelli, Maria Teresa Conconi, Pier Paolo Parnigotto
• Journal: Journal of cellular and molecular medicine (J Cell Mol Med) Vol. 22 Issue 3 Pg. 1840-1854 (03 2018) ISSN: 1582-4934 [Electronic] England
• PMID: 29314633 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Chemical References

• Intercellular Signaling Peptides and Proteins

Topics

• Adipogenesis (drug effects, genetics)
• Cell Differentiation (drug effects, genetics)
• Cell Proliferation (drug effects, genetics)
• Cells, Cultured
• Fibroblasts (cytology, drug effects, metabolism)
• Gene Expression Profiling (methods)
• Humans
• Intercellular Signaling Peptides and Proteins (metabolism, pharmacology)
• Leukocytes (metabolism)
• Multipotent Stem Cells (cytology, drug effects, metabolism)
• Neurogenesis (drug effects, genetics)
• Platelet-Rich Fibrin (metabolism)
• Proteomics (methods)
• Regenerative Medicine (methods)
• Wound Healing (drug effects)