ScFv-modified graphene-coated IDE-arrays for 'label-free' screening of cardiovascular disease biomarkers in physiological saline.

Abstract

Fatty-acid binding proteins (FABP) and myeloperoxidases (MPO) are associated with many chronic conditions in humans and considered to be important biomarkers for diagnosis of cardiac diseases. Here we assemble a new electrical biosensor platform based on graphene-coated interdigitated electrode arrays (IDE-arrays) towards ultrafast, label-free screening of heart type-FABP and MPO. Arrays of nanoscale (nanoIDE) and microscale (microIDE) electrode-arrays were fabricated on wafer-scale by combining nanoimprint and photolithography processes. Chemical vapor deposition grown multilayer graphene was transferred onto nano/microIDE-arrays and used as a high surface-to-volume ratio electrical transducer. Novel biofunctional layers of specially engineered anti-h-FABP and anti-MPO single-chain fragment variables (scFv) were immobilized onto graphene-coated IDE-array sensor platform for electrical detection of h-FABP and MPO in physiological saline. scFv fragments show increased sensitivity in comparison to the state-of-the-art competitive ELISA for their higher affinity towards target analytes. Deploying FABP and MPO specific scFvs as receptor molecules onto our high-sensitivity graphene-coated IDE-arrays with identical sensor characteristics and assays covering clinically relevant concentrations in physiological saline, we demonstrate realization of a simple and versatile biosensor platform capable of high performance cardiac-bioassays for point-of-care applications.

• Authors: Lotta E Delle, Vivek Pachauri, Shikha Sharma, Olena Shaforost, Hui Ma, Mohammad Adabi, Rainer Lilischkis, Patrick Wagner, Ronald Thoelen, Norbert Klein, Richard O'Kennedy, Sven Ingebrandt
• Journal: Biosensors & bioelectronics (Biosens Bioelectron) Vol. 102 Pg. 574-581 (Apr 15 2018) ISSN: 1873-4235 [Electronic] England
• PMID: 29241061 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier B.V. All rights reserved.

Chemical References

• Biomarkers
• Fatty Acid Binding Protein 3
• Interleukin-3
• Recombinant Fusion Proteins
• Single-Chain Antibodies
• myelopoietin
• Granulocyte Colony-Stimulating Factor
• Graphite

Topics

• Biomarkers (blood)
• Biosensing Techniques (methods)
• Cardiovascular Diseases (blood)
• Fatty Acid Binding Protein 3 (blood, immunology, isolation & purification)
• Granulocyte Colony-Stimulating Factor (blood, immunology, isolation & purification)
• Graphite (chemistry)
• Humans
• Interleukin-3 (blood, immunology, isolation & purification)
• Recombinant Fusion Proteins (blood, immunology, isolation & purification)
• Single-Chain Antibodies (chemistry, immunology)