Cervical cancer is one of the most common female
malignancies worldwide. Emerging data have shown that
microRNAs (
miRNAs) play significant roles in various human
cancers, including
cervical cancer. Aberrantly expressed
miRNAs in
cervical cancer contribute to tumour occurrence and development as either tumour suppressors or promoters. Research suggests that miRNA-433 (miR-433) possibly plays an important role in the development of various
cancer types. However, no study has explored the expression patterns, roles and underlying mechanisms of miR-433 in
cervical cancer. In the present study, we demonstrated significant downregulation of miR-433 in
cervical cancer tissues and cell lines. Low miR-433 expression was found to significantly correlate with patient characteristics including tumour size, International Federation of Gynecology and Obstetrics stage, lymph node and distant
metastases. Functional studies showed that restoration of miR-433 inhibited cell proliferation and invasion and increased apoptosis in
cervical cancer cells. Metadherin (MTDH) was also validated as a direct target gene of miR-433. MTDH
mRNA expression was upregulated in
cervical cancer tissues and was inversely correlated with miR-433 expression. MTDH knockdown showed similar tumour-suppressive roles as miR-433 overexpression in regards to
cervical cancer cell proliferation, invasion and apoptosis. Rescue experiments revealed that MTDH overexpression markedly reversed the effects of miR-433 overexpression in regards to proliferation, invasion and apoptosis of
cervical cancer cells. Further investigations revealed that miR-433 inactivated AKT and β-
catenin pathways in
cervical cancer. Collectively, these findings indicate the essential roles of miR-433 in suppressing
cervical cancer progression and suggest its potential as a therapeutic target for the treatment of
cervical cancer.