Abstract |
Glycoprotein nonmetastatic melanoma protein B (GPNMB) has a neuroprotective effect against neuronal cell death caused by the accumulation of abnormal mutated proteins. It is known that the accumulation of pathological proteins induces endoplasmic-reticulum (ER) stress leading to cell damage. The aim of this study was to determine the role of GPNMB in the ER stress response. GPNMB was greatly up-regulated by thapsigargin-induced ER stress. Under the ER stress conditions, GPNMB relocated to the nucleus and specifically up-regulated expression of BiP at the mRNA level by promoting the BiP pre-mRNA splicing, not through the pathways initiated by the three major transducers of the unfolded protein response: IRE1, PERK, and ATF6. Furthermore, we found that the protein level of BiP and the infarction were increased and attenuated, respectively, in Gpnmb-transgenic mice after occlusion of the middle cerebral artery, in comparison with wild-type mice. Thus, our findings indicate that GPNMB enhances the BiP expression by promoting the splicing (thereby preventing cell death caused by ER stress) and could be a therapeutic target in ER stress-related disorders.
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Authors | Yasuhiro Noda, Kazuhiro Tsuruma, Masafumi Takata, Mitsue Ishisaka, Hirotaka Tanaka, Yusuke Nakano, Yuki Nagahara, Masamitsu Shimazawa, Hideaki Hara |
Journal | Scientific reports
(Sci Rep)
Vol. 7
Issue 1
Pg. 12160
(09 22 2017)
ISSN: 2045-2322 [Electronic] England |
PMID | 28939899
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Endoplasmic Reticulum Chaperone BiP
- Eye Proteins
- Gpnmb protein, mouse
- Heat-Shock Proteins
- Membrane Glycoproteins
- RNA Precursors
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Topics |
- Animals
- Cell Line
- Endoplasmic Reticulum Chaperone BiP
- Endoplasmic Reticulum Stress
- Eye Proteins
(metabolism)
- Heat-Shock Proteins
(genetics)
- Infarction, Middle Cerebral Artery
(genetics, metabolism)
- Membrane Glycoproteins
(metabolism)
- Mice, Transgenic
- Protein Transport
- RNA Precursors
(genetics)
- RNA Splicing
- Up-Regulation
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