Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status.

Abstract

The type V intermediate filament lamins are the principal components of the nuclear matrix, including the nuclear lamina. Lamins are divided into A-type and B-type, which are encoded by three genes, LMNA, LMNB1, and LMNB2. The alternative splicing of LMNA produces two major A-type lamins, lamin A and lamin C. Previous studies have suggested that lamins are involved in cancer development and progression. A-type lamins have been proposed as biomarkers for cancer diagnosis, prognosis, and/or follow-up. The aim of the present study was to investigate lamins in cancer cells from metastatic pleural effusions using immunofluorescence, western blotting, and flow cytometry. In a sub-group of lung adenocarcinomas, we found reduced expression of lamin A but not of lamin C. The reduction in lamin A expression was correlated with the loss of epithelial membrane antigen (EMA)/MUC-1, an epithelial marker that is involved in the epithelial to mesenchymal transition (EMT). Finally, the lamin A expression was inversely correlated with the number of metastatic sites and the WHO Performance status, and association of pleural, bone and lung metastatic localizations was more frequent when lamin A expression was reduced. In conclusion, low lamin A but not lamin C expression in pleural metastatic cells could represent a major actor in the development of metastasis, associated with EMT and could account for a pejorative factor correlated with a poor Performance status.

• Authors: Elise Kaspi, Diane Frankel, Julien Guinde, Sophie Perrin, Sophie Laroumagne, Andrée Robaglia-Schlupp, Kevin Ostacolo, Karim Harhouri, Rachid Tazi-Mezalek, Joelle Micallef, Hervé Dutau, Pascale Tomasini, Annachiara De Sandre-Giovannoli, Nicolas Lévy, Pierre Cau, Philippe Astoul, Patrice Roll
• Journal: PloS one (PLoS One) Vol. 12 Issue 8 Pg. e0183136 ( 2017) ISSN: 1932-6203 [Electronic] United States
• PMID: 28806747 (Publication Type: Journal Article)

Chemical References

• Lamin Type A
• Mucin-1
• lamin C

Topics

• Adenocarcinoma (metabolism, pathology)
• Adenocarcinoma of Lung
• Aged
• Aged, 80 and over
• Female
• Flow Cytometry
• Fluorescent Antibody Technique
• Humans
• Lamin Type A (metabolism)
• Lung Neoplasms (metabolism, pathology)
• Male
• Middle Aged
• Mucin-1 (metabolism)
• Neoplasm Metastasis
• Pleural Effusion (metabolism, pathology)
• World Health Organization