Abstract | BACKGROUND: The persistence of eosinophils in sputum despite high doses of corticosteroids indicates disease severity in asthmatic patients. Chronic inflamed airways can lose tolerance over time to immunogenic entities released on frequent eosinophil degranulation, which further contributes to disease severity and necessitates an increase in maintenance corticosteroids. OBJECTIVES: We sought to investigate the possibility of a polyclonal autoimmune event in the airways of asthmatic patients and to identify associated clinical and molecular characteristics. METHODS: RESULTS: We report a "polyclonal" autoimmune event occurring in the airways of prednisone-dependent asthmatic patients with increased eosinophil activity, recurrent pulmonary infections, or both, as evident by the concomitant presence of sputum anti-EPX and anti-nuclear antibodies of the IgG subtype. Extensive cytokine profiling of sputum revealed a TH2-dominated microenvironment (eotaxin-2, IL-5, IL-18, and IL-13) and increased signalling molecules that support the formation of ectopic lymphoid structures ( B-cell activating factor and B cell-attracting chemokine 1). Immunoprecipitated sputum immunoglobulins from patients with increased autoantibody levels triggered eosinophil degranulation in vitro, with release of extensive histone-rich extracellular traps, an event unsuppressed by dexamethasone and possibly contributing to the steroid-unresponsive nature of these eosinophilic patients. CONCLUSION: This study identifies an autoimmune endotype of severe asthma that can be identified by the presence of sputum autoantibodies against EPX and autologous cellular components.
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Authors | Manali Mukherjee, David C Bulir, Katherine Radford, Melanie Kjarsgaard, Chynna Margaret Huang, Elizabeth A Jacobsen, Sergei I Ochkur, Ana Catuneanu, Hanah Lamothe-Kipnes, James Mahony, James J Lee, Paige Lacy, Parameswaran K Nair |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 141
Issue 4
Pg. 1269-1279
(04 2018)
ISSN: 1097-6825 [Electronic] United States |
PMID | 28751233
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2017 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Anti-Asthmatic Agents
- Antibodies, Antinuclear
- Autoantibodies
- Biomarkers
- Eosinophil Peroxidase
- Prednisone
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Anti-Asthmatic Agents
(therapeutic use)
- Antibodies, Antinuclear
(metabolism)
- Asthma
(drug therapy, immunology)
- Autoantibodies
(metabolism)
- Biomarkers
(metabolism)
- Eosinophil Peroxidase
(immunology)
- Female
- Humans
- Male
- Middle Aged
- Prednisone
(therapeutic use)
- Pulmonary Eosinophilia
(drug therapy, immunology)
- Retrospective Studies
- Severity of Illness Index
- Sputum
(immunology)
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