Abstract | OBJECTIVE: DESIGN: Fifty male Sprague Dawley rats were randomly divided into the following five groups: (1) a control group (saline placebo without ligature and STZ injection), (2) a PTH group (PTH administration without ligature and STZ injection), (3) an L group (saline placebo with ligature), (4) an L+STZ group (saline placebo with ligature and STZ injection), and (5) an L+STZ+PTH group (PTH administration with ligature and STZ injection). PTH was administered at 75μg/kg per dose four times a week for 28days. Subsequently, all rats were sacrificed, and their mandibles were extracted for micro-computed tomography (micro-CT) scanning, as well as histological and immunochemical evaluation. RESULTS: Micro-CT scanning demonstrated the anabolic effect of PTH on alveolar bone metabolism in STZ-induced diabetic rats (P<0.05), and histomorphometry indicated that PTH inhibited inflammation of the periodontium and increased the level of osteoblastic activity (P<0.05). Immunochemical evaluation showed that rats subjected to both ligature placement and STZ injection had the highest receptor activator of nuclear factor kappa B ligand (RANKL)/ osteoprotegerin (OPG) ratio and that PTH administration decreased this ratio. CONCLUSION: Intermittent systemic PTH administration effectively reduced alveolar bone loss and ameliorated the manifestation of experimental periodontitis in STZ-induced diabetic rats.
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Authors | Helin Chen, Taozi Fu, Yuanyuan Ma, Xiangnan Wu, Xianxian Li, Xinyi Li, Jiefei Shen, Hang Wang |
Journal | Archives of oral biology
(Arch Oral Biol)
Vol. 83
Pg. 76-84
(Nov 2017)
ISSN: 1879-1506 [Electronic] England |
PMID | 28732226
(Publication Type: Journal Article)
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Copyright | Copyright © 2017 Elsevier Ltd. All rights reserved. |
Chemical References |
- Osteoprotegerin
- Parathyroid Hormone
- RANK Ligand
- Streptozocin
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Topics |
- Alveolar Bone Loss
(diagnostic imaging, prevention & control)
- Animals
- Diabetes Mellitus, Experimental
(complications)
- Male
- Maxilla
(diagnostic imaging)
- Osteoblasts
(drug effects)
- Osteoclasts
(drug effects)
- Osteoprotegerin
(metabolism)
- Parathyroid Hormone
(administration & dosage, pharmacology)
- Periodontitis
(diagnostic imaging, etiology, prevention & control)
- RANK Ligand
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Streptozocin
- X-Ray Microtomography
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