ELISA analysis revealed significantly increased levels in both AqH and vitreous of ATF4 and
GRP78 in eyes affected with PDR compared to the controls (all p < 0.001). The mean concentrations of
IL-6, MCP-1 were also higher in both AqH and vitreous samples from patients with PDR compared to those of IMH (all p < 0.001). (Independent Student t-test, normality test followed with Skewness-Kurtosis Test). In addition, correlations of ATF4 and
GRP78 with inflammatory factors
IL-6 and MCP-1 in subjects of patients were analyzed. No significant correlation between the AqH concentrations of ATF4/IL-6 and ATF4/MCP-1 was detected in eyes of PDR patients (r = 0.346, p = 0.072 and r = 0.275, p = 0.157). Significant correlations were observed between AqH concentrations of
GRP78/IL-6 (r = 0.724, p < 0.001),
GRP78/MCP-1 (r = 0.654, p < 0.001) in PDR patients. Significant correlations were observed between vitreous concentrations of ATF4/IL-6 (r = 0.918, p < 0.001), ATF4/MCP-1 (r = 0.921, p < 0.001),
GRP78/IL-6 (r = 0.978, p < 0.001),
GRP78/MCP-1 (r = 0.979, p < 0.001) in PDR patients. No significant correlations was observed between AqH concentrations of ATF4/IL-6 (r = 0.187, p = 474), ATF4/MCP-1 (r = 0.240, p = 0.353),
GRP78/IL-6 (r = 0.321, p = 0.209) and
GRP78/MCP-1 (r = 0.169, p = 0.516) in eyes of IMH patients. And also no significant correlation was observed between vitreous concentrations of ATF4/IL-6 (r = 0.130, p = 0.563), ATF4/MCP-1(r = 0.029, p = 0.897),
GRP78/IL-6 (r = 0.078, p = 0.717),
GRP78/MCP-1 (r = 0.005, p = 0.982) in IMH patients. (Pearson correlation coefficient (two-tailed)).
CONCLUSIONS: