[Effect of CDK Inhibitor LS-007 on Acute Lymphoblastic Leukemia and Its Mechanism].

Abstract

OBJECTIVE:

To study the effect of cyclin dependent kinase(CDK) inhibitor LS-007 on acute lymphoblastic leukemia and its mechanism.

METHODS:

The acute lymphocytic leukemia cell line was cultured and treated by LS-007, flavopiridol and ABT-199, then the changes of apoptosis-related factor mRNA and protein levels were detected by using mRNA quantitative PCR and Werstern blot.

RESULTS:

quantitative PCR and Western blot detection showed that the levels of antiapoptotic protein decreased significantly in acute lymphoblastic leukemia cells after LS-007 treatment, and the pro-apoptotic effect of LS-007 combined with ABT-199 was much better.

CONCLUSION:

LS-007 can affect the phosphorylation of RNA polymerase sites and promote cell apoptosis through changing the activities of CDK, thus having some positive significance for relieving acute lymphoblastic leukemia.

• Authors: Jiang-Feng Lu, Feng-Ling Xu, Jun Lu, Yu-Guo Ren
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 25 Issue 2 Pg. 393-397 (Apr 2017) ISSN: 1009-2137 [Print] China
• PMID: 28446281 (Publication Type: Journal Article)

Chemical References

• 3-(5-fluoro-4-(4-methyl-2-(methylamino)thiazol-5-yl)pyrimidin-2-ylamino)benzenesulfonamide
• Pyrimidines
• Sulfonamides
• Cyclin-Dependent Kinases

Topics

• Apoptosis
• Cell Line, Tumor
• Cyclin-Dependent Kinases
• Humans
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (drug therapy)
• Pyrimidines (pharmacology)
• Sulfonamides (pharmacology)
• Tumor Cells, Cultured