Quantitative analysis of Shigella flexneri protein expression under acid stress.

Abstract

As an important foodborne pathogen, Shigella flexneri can cause widespread enteric infection with bacteria as few as hundreds. This is, at least in part, attributed to its robust anti-acid strategies because passage through the highly acidic human digestive tract is a prerequisite for successful bacterial infection. Nevertheless, our understanding of these mechanisms and the impact of acid stress on Shigella protein expression still remains largely incomplete. Herein we conducted a proteomic survey of Shigella spp. under acid stress. Out of 1754 protein identifications, we found 131 altered proteins, most of which were down-regulated, including virulence factors and cell envelope proteins. Rather, many metabolic enzymes and pyrimidine/amino acid biosynthesis proteins were up-regulated. In addition to induction of many known anti-acid systems, we also found marked increase of 2-oxoglutarate dehydrogenase (SucAB), a metabolic enzyme in the tricarboxylic acid cycle. Importantly, overproduction of this enzyme significantly enhanced Shigella acid resistance and hence SucAB-mediated metabolic pathways may represent novel anti-acid strategies.

• Authors: Kaiwen Yu, Zhen Wang, Fan Zhou, Jiezhang Jiang, Yanhua Liu, Mo Hu, Xiaoyun Liu
• Journal: Proteomics (Proteomics) Vol. 17 Issue 8 (Apr 2017) ISSN: 1615-9861 [Electronic] Germany
• PMID: 28221718 (Publication Type: Journal Article)
• Copyright: © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Bacterial Proteins
• Ketoglutarate Dehydrogenase Complex

Topics

• Bacterial Proteins (analysis, metabolism)
• Chromatography, Liquid
• Citric Acid Cycle
• Hydrogen-Ion Concentration
• Ketoglutarate Dehydrogenase Complex (metabolism)
• Metabolic Networks and Pathways
• Proteomics (methods)
• Shigella flexneri (chemistry, metabolism, pathogenicity)
• Stress, Physiological (physiology)
• Tandem Mass Spectrometry