First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-κB in Living Cells.

Abstract

NF-κB is a critical transcription factor that plays an important role in mediating inflammation, the immune response, and cell proliferation. The activation of NF-κB leads to an enhancement of proinflammatory mediator expression, which is implicated in the pathogenesis of a variety of diseases. Therefore, methods that allow the intracellular tracking of NF-κB are particularly attractive because they can provide information regarding the pathways or stimulation responses that are involved in the activation of NF-κB. In this work, we report a novel platform to track intracellular NF-κB by employing the conjugated iridium(III) complex 1, which was synthesized through the unique combination of a luminescent iridium(III) moiety with the natural product oridonin. Experiments conducted with p50 knockdown cells revealed that complex 1 could detect the p50 subunit of NF-κB in cellulo. Furthermore, complex 1 tracked NF-κB translocation induced by tumor necrosis factor-α (TNF-α) as a model stimulus, without affecting the translocation process itself. To the best of our knowledge, complex 1 is the first metal-based compound that has been reported to be capable of monitoring intracellular NF-κB in living cells.

• Authors: Wanhe Wang, Chao Yang, Sheng Lin, Kasipandi Vellaisamy, Guodong Li, Weihong Tan, Chung-Hang Leung, Dik-Lung Ma
• Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (Chemistry) Vol. 23 Issue 20 Pg. 4929-4935 (Apr 06 2017) ISSN: 1521-3765 [Electronic] Germany
• PMID: 28211965 (Publication Type: Journal Article)
• Copyright: © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Coordination Complexes
• Diterpenes, Kaurane
• NF-kappa B
• NF-kappa B p50 Subunit
• Tumor Necrosis Factor-alpha
• oridonin
• Iridium

Topics

• Coordination Complexes (chemical synthesis, chemistry, metabolism)
• Diterpenes, Kaurane (chemistry)
• HeLa Cells
• Humans
• Iridium (chemistry)
• Microscopy, Fluorescence
• NF-kappa B (metabolism)
• NF-kappa B p50 Subunit (deficiency, genetics)
• Transcriptional Activation (drug effects)
• Tumor Necrosis Factor-alpha (pharmacology)