Abstract |
Differential mRNA polyadenylation plays an important role in shaping the neuronal transcriptome. In C. elegans, several ankyrin isoforms are produced from the unc-44 locus through alternative polyadenylation. Here, we identify a key role for an intronic polyadenylation site (PAS) in temporal- and tissue-specific regulation of UNC-44/ ankyrin isoforms. Removing an intronic PAS results in ectopic expression of the neuronal ankyrin isoform in non-neural tissues. This mis-expression underlies epidermal developmental defects in mutants of the conserved tumor suppressor death-associated protein kinase dapk-1 We have previously reported that the use of this intronic PAS depends on the nuclear polyadenylation factor SYDN-1, which inhibits the RNA polymerase II CTD phosphatase SSUP-72. Consistent with this, loss of sydn-1 blocks ectopic expression of neuronal ankyrin and suppresses epidermal morphology defects of dapk-1 These effects of sydn-1 are mediated by ssup-72 autonomously in the epidermis. We also show that a peptidyl- prolyl isomerase PINN-1 antagonizes SYDN-1 in the spatiotemporal control of neuronal ankyrin isoform. Moreover, the nuclear localization of PINN-1 is altered in dapk-1 mutants. Our data reveal that tissue and stage-specific expression of ankyrin isoforms relies on differential activity of positive and negative regulators of alternative polyadenylation.
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Authors | Fei Chen, Andrew D Chisholm, Yishi Jin |
Journal | Development (Cambridge, England)
(Development)
Vol. 144
Issue 4
Pg. 698-707
(02 15 2017)
ISSN: 1477-9129 [Electronic] England |
PMID | 28087624
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2017. Published by The Company of Biologists Ltd. |
Chemical References |
- Ankyrins
- Caenorhabditis elegans Proteins
- Protein Isoforms
- RNA, Messenger
- Green Fluorescent Proteins
- Death-Associated Protein Kinases
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Topics |
- Animals
- Ankyrins
(genetics, metabolism)
- Caenorhabditis elegans
(embryology)
- Caenorhabditis elegans Proteins
(metabolism)
- Cell Nucleus
(metabolism)
- Death-Associated Protein Kinases
(metabolism)
- Epidermis
(embryology)
- Gene Expression Profiling
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins
(metabolism)
- Introns
- Mutation
- Neurons
(metabolism)
- Phenotype
- Polyadenylation
- Protein Isoforms
- RNA, Messenger
(metabolism)
- Tissue Distribution
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