Abstract | BACKGROUND: Development of new apoptosis-inducing drugs is a promising trend in anticancer therapy. For this purpose several formamidinoderivatives of doxorubicin were synthesized. The aim of our study was to investigate effects of the five formamidinodoxorubicins in the ES-2 human ovarian clear cell carcinoma line, for comparison with data obtained previously for SKOV-3 human ovarian adenocarcinoma cells, to answer the question of whether and to what extent the histological cell type is a possible determinant of sensitivity to tested anthracyclines. MATERIALS AND METHODS: RESULTS: Effects of the derivatives of doxorubicin were partially linked with the specific type of cancer cell although intracellular accumulation and cellular uptake of DOX and derivatives were similar in both. All of the investigated derivatives were considerably more cytotoxic than DOX. Formamidinodoxorubicins were able to induce caspase-dependent apoptotic cell death in both cell types. CONCLUSIONS: All new formamidine derivatives of DOX were able to induce caspase - dependent apoptosis in human ovarian cancer cell lines SKOV-3 and ES-2. Obtained results suggested that formamidine derivatives of DOX may be promising candidates for the prospective chemotherapeutic agents for the two different histological subtypes of ovarian cancer.
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Authors | M Denel-Bobrowska, M Lukawska, I Oszczapowicz, A Marczak |
Journal | Asian Pacific journal of cancer prevention : APJCP
(Asian Pac J Cancer Prev)
Vol. 17
Issue 9
Pg. 4223-4231
( 2016)
ISSN: 2476-762X [Electronic] Thailand |
PMID | 27797222
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Amidines
- Antibiotics, Antineoplastic
- formamidine
- Doxorubicin
- Caspases
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Topics |
- Adenocarcinoma, Clear Cell
(drug therapy, metabolism, pathology)
- Amidines
(chemistry)
- Antibiotics, Antineoplastic
(chemistry, pharmacology)
- Apoptosis
(drug effects)
- Caspases
(metabolism)
- Cell Proliferation
(drug effects)
- Doxorubicin
(chemistry, pharmacology)
- Drug Resistance, Neoplasm
- Female
- Humans
- In Vitro Techniques
- Membrane Potential, Mitochondrial
(drug effects)
- Microscopy, Fluorescence
- Ovarian Neoplasms
(drug therapy, metabolism, pathology)
- Tumor Cells, Cultured
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