[Effect of hypobaric hypoxic preconditioning on surgically induced endometriosis by allotransplant of uterine tissue in rats].

Abstract

OBJECTIVE:

To determine the effects of hypobaric hypoxia pretreatment on surgically induced endometriosis in rats.

METHODS:

Six rats were randomized into 2 groups and exposed to hypoxia (8% O2) and normoxia (21% O2) for 8 h. The uterine endometrium was intraperitoneally implanted into estrogen-treated ovariectomized Lewis rat, and the growth and quality of the implants were measured. The changes in apoptosis, protein and gene expressions in the serum, abdominis effusion fluids and implants were tested by ELISA, immunohistochemical staining, TUNNEL assay, Western blotting and RT-PCR.

RESULTS:

The volume of the implants in the hypoxic pretreatment group was significantly increased compared with the normoxia group. High expressions of Ki67, CD31, VEGF, and HIF-1α and lowered cell apoptosis were found in the hypoxia-pretreated implants compared with the normoxic group. VEGF level in the serum and peritoneal fluid were increased in hypoxia-pretreated group, but TNFα level was comparable between the 2 groups.

CONCLUSION:

Hypoxia play an important role in the occurrence and progression of endometriosis by increasing cell proliferation and angiogenesis and decreasing cell apoptosis in the implants in the rat model.

• Authors: Jun Zhao, Xiao Liu, Ya-Li Li, Ming-Hua Tang
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 36 Issue 10 Pg. 1369-1376 (Oct 20 2016) ISSN: 1673-4254 [Print] China
• PMID: 27777200 (Publication Type: Journal Article)

Chemical References

• Hif1a protein, rat
• Hypoxia-Inducible Factor 1, alpha Subunit
• Vascular Endothelial Growth Factor A
• vascular endothelial growth factor A, rat

Topics

• Allografts
• Animals
• Apoptosis
• Ascitic Fluid
• Blotting, Western
• Cell Proliferation
• Endometriosis (therapy)
• Endometrium (transplantation)
• Female
• Hypoxia
• Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
• Ischemic Preconditioning
• Neovascularization, Pathologic
• Rats
• Rats, Inbred Lew
• Vascular Endothelial Growth Factor A (blood)