Chicken IL-7 as a potent adjuvant enhances IBDV VP2 DNA vaccine immunogenicity and protective efficacy.

Abstract

Our previous work has demonstrated that the mammalian interleukin-7 (IL-7) gene can enhance the immunogenicity of DNA vaccine. Whether chicken IL-7 (chIL-7) possesses the ability to enhance the immunogenicity of VP2 DNA vaccine of infectious bursal disease virus (IBDV) remained unknown. To investigate this, we constructed a VP2 antigenic region (VP2366) gene and chIL-7 gene vectors, co-immunized chicken with these vectors and analyzed the effects of the chIL-7 gene on VP2366 gene immunogenicity. Results showed that co-administrated chIL-7 gene with VP2 DNA vaccine significantly increased specific serum antibody titers against IBDV, and enhanced lymphocyte proliferation and IFN-γ and IL-4 productions. More importantly, chIL-7 gene significantly increased VP2366 gene-induced protection against virulent IBDV infection, indicating that the chIL-7 gene possessed the capacity to enhance VP2366 DNA vaccine immunogenicity, and therefore might function as a novel adjuvant for IBDV VP2 DNA vaccine. Mechanically, chIL-7 could stimulate the common cytokine receptor γ chain (γc) expressions in vitro and in vivo, which might be involved in chIL-7 enhancement of the immunogenicity of VP2 DNA vaccine.

• Authors: Shanshan Huo, Yuzhu Zuo, Nan Li, Xiujin Li, Yonghong Zhang, Liyue Wang, Hao Liu, Jianlou Zhang, Dan Cui, Pingyou He, Jian Xu, Yan Li, Xiutong Zhu, Fei Zhong
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 193 Pg. 145-55 (Sep 25 2016) ISSN: 1873-2542 [Electronic] Netherlands
• PMID: 27599941 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Interleukin-7
• VP2 protein, infectious bursal disease virus
• Vaccines, DNA
• Viral Structural Proteins
• Interleukin-4
• Interferon-gamma

Topics

• Adjuvants, Immunologic (administration & dosage)
• Amino Acid Sequence
• Animals
• Base Sequence
• Birnaviridae Infections (immunology, prevention & control, veterinary, virology)
• Chickens (immunology)
• Immunization
• Immunogenicity, Vaccine
• Infectious bursal disease virus (genetics, immunology)
• Interferon-gamma (immunology)
• Interleukin-4 (immunology)
• Interleukin-7 (administration & dosage)
• Poultry Diseases (immunology, prevention & control, virology)
• Sequence Analysis, DNA (veterinary)
• Vaccines, DNA (administration & dosage)
• Viral Structural Proteins (genetics, immunology)