CK2, a
protein serine/threonine kinase, promotes cell proliferation and suppresses cell death. This essential-for-survival signal demonstrates elevated expression and activity in all
cancers examined, and is considered an attractive target for
cancer therapy. Here, we present data on the efficacy of a
tenfibgen (TBG) coated
nanocapsule which delivers its cargo of
siRNA (siCK2) or single stranded
RNA/
DNA oligomers (RNAi-CK2) simultaneously targeting CK2α and α' catalytic subunits.
Intravenous administration of TBG-siCK2 or TBG-RNAi-CK2 resulted in significant xenograft
tumor reduction at low doses in PC3-LN4 and 22Rv1 models of
prostate cancer. Malignant cell uptake and specificity in vivo was verified by FACS analysis and immunofluorescent detection of
nanocapsules and PCR detection of released oligomers. Dose response was concordant with CK2αα'
RNA transcript levels and the
tumors demonstrated changes in CK2
protein and in markers of proliferation and cell death. Therapeutic response corresponded to expression levels for argonaute and GW
proteins, which function in oligomer processing and translational repression. No toxicity was detected in non-
tumor tissues or by serum chemistry.
Tumor specific delivery of anti-CK2 RNAi via the TBG nanoencapsulation technology warrants further consideration of translational potential.