Proximal CD13 Versus Distal GATA-3 Expression in Renal Neoplasia According to WHO 2016 Classification.

Abstract

Little is known about the aminopeptidase CD13 in renal neoplasia according to the new 2016 World Health Organization renal tumor classification. We selected 175 cases, including 79 clear cell, 31 papillary, 24 chromophobe, 8 clear cell papillary renal cell carcinomas (RCCs), 21 oncoytomas, and 12 microphthalmia transcription factor family translocation RCCs: 4 t(6;11)/transcription factor EB (TFEB), 7 t(Xp11) with 2 cystic variants and 1 t(X;17). GATA binding protein 3 (GATA-3) was inserted as control. Expression of proximal antigen CD13 was observed in 63/79 (80%) clear cell, 25/31 (81%) papillary, 3/8 (37%) clear cell papillary, 1/4 (25%) t(6;11)/TFEB, 2/7 (28%) cystic t(Xp11), and in 1/1 t(X;17) RCCs. All chromophobe RCC (0/24) and all oncocytomas (0/21) resulted negative. CD10 was seen in 76/79 (96%) clear cell, 15/31 (48%) papillary, 10/24 (42%) chromophobe, 1/8 (12%) clear cell papillary RCCs, 4/21 (19%) oncocytomas, 1/4 (25%) t(6;11)/TFEB, 2/7 (29%) cystic t(Xp11), and in 1/1 t(X;17) RCCs. GATA-3 was positive in 3/7 (42%) clear cell papillary RCCs and negative in all remaining RCCs, except a single chromophobe RCC and a single oncocytoma. We concluded that: (1) CD13 and GATA-3 immunostains may serve as a diagnostic aid in differentiating subtypes of RCC; (2) CD13 is always absent in chromophobe RCC and oncocytomas, whereas CD10 can be immunoexpressed in both; (3) CD13 should be included in a panel of antibodies to distinguish "proximal renal tumors" from "distal renal tumors" and between clear cell RCC versus microphthalmia transcription factor family translocations RCCs; and (4) when present, GATA-3 is specific for clear cell papillary RCC.

• Authors: Matteo Brunelli, Francesco Erdini, Luca Cima, Albino Eccher, Adele Fioravanzo, Stefano Gobbo, Diego Segala, Claudio Ghimenton, Guido Mazzoleni, Enrico Munari, Rodolfo Carella, Guido Martignoni
• Journal: Applied immunohistochemistry & molecular morphology : AIMM (Appl Immunohistochem Mol Morphol) 2018 May/Jun Vol. 26 Issue 5 Pg. 316-323 ISSN: 1533-4058 [Electronic] United States
• PMID: 27556823 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
• Biomarkers, Tumor
• GATA3 Transcription Factor
• GATA3 protein, human
• TFEB protein, human
• CD13 Antigens

Topics

• Adenoma, Oxyphilic (diagnosis)
• Basic Helix-Loop-Helix Leucine Zipper Transcription Factors (genetics)
• Biomarkers, Tumor (metabolism)
• CD13 Antigens (metabolism)
• Carcinoma, Papillary (diagnosis)
• Carcinoma, Renal Cell (diagnosis)
• Diagnosis, Differential
• GATA3 Transcription Factor (metabolism)
• Humans
• Immunochemistry
• Kidney Neoplasms (diagnosis)
• Male
• Translocation, Genetic
• World Health Organization