UVB exposure enhanced benzanthrone-induced inflammatory responses in SKH-1 mouse skin by activating the expression of COX-2 and iNOS through MAP kinases/NF-κB/AP-1 signalling pathways.

Abstract

This study was conducted to explore the role of UVB on benzanthrone (BA)-induced skin inflammation and its mechanism/s. SKH-1 hairless mice were topically exposed with BA (25 and 50 mg/kg b.wt) either alone or along with UVB (50 mJ/cm(2)) for 24 h and estimation of ROS, histopathological analysis, myeloperoxidase (MPO) activity, mast cell staining, immunohistochemistry for COX-2 and iNOS as well as western blotting for MAPKs, p-NF-κB, c-jun, c-fos COX-2 and iNOS were carried out. Enhanced ROS generation, increased epidermal thickness, mast cell number, MPO activity, enhanced expression of COX-2 and iNOS, MAPKs, c-jun, c-fos, NF-κB were found in BA either alone or when followed by UVB treatment, compared to the control groups. Expression of COX-2, iNOS and phosphorylation of ERK1/2 were found to be more enhanced in BA and UVB- exposed group compared to BA and UVB only group, while phosphorylation of JNK1/2, p38, NF-κB and expression of c-jun and c-fos were comparable with BA and UVB only groups. In summary, we suggest that UVB exposure enhanced BA-induced SKH-1 skin inflammation possibly via oxidative stress-mediated activation of MAPKs-NF-κB/AP-1 signalling, which subsequently increased the expression of COX-2 and iNOS and led to inflammation in SKH-1 mouse skin.

• Authors: Sabiya Abbas, Shamshad Alam, Anu Pal, Mahadeo Kumar, Dhirendra Singh, Kausar Mahmood Ansari
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 96 Pg. 183-90 (Oct 2016) ISSN: 1873-6351 [Electronic] England
• PMID: 27507225 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Ltd. All rights reserved.

Chemical References

• Benz(a)Anthracenes
• NF-kappa B
• Reactive Oxygen Species
• Transcription Factor AP-1
• NOS2 protein, human
• Nitric Oxide Synthase Type II
• Cyclooxygenase 2
• Mitogen-Activated Protein Kinases
• benzanthrone

Topics

• Animals
• Benz(a)Anthracenes (toxicity)
• Cyclooxygenase 2 (metabolism)
• Female
• Immunoblotting
• Immunoenzyme Techniques
• Inflammation (etiology, metabolism, pathology)
• Lipid Peroxidation (drug effects)
• Mice
• Mice, Hairless
• Mitogen-Activated Protein Kinases (metabolism)
• NF-kappa B (metabolism)
• Nitric Oxide Synthase Type II (metabolism)
• Oxidative Stress (drug effects)
• Phosphorylation (drug effects)
• Reactive Oxygen Species (metabolism)
• Signal Transduction (drug effects)
• Transcription Factor AP-1 (metabolism)
• Ultraviolet Rays (adverse effects)