Abstract | BACKGROUND/AIMS: Despite significant advancements in the diagnosis and treatment of osteosarcoma, the overall survival has remained relatively unchanged for over two decades. Hypoxic conditions have been demonstrated in solid tumors and are associated with increased cell proliferation and angiogenesis. L-arginine metabolism by arginase produces L- ornithine, the precursor for polyamine and proline synthesis required for cellular proliferation. We hypothesized that hypoxia would increase cellular proliferation via arginase induction in human osteosarcoma cell lines. METHODS: We utilized a variety of approaches to examine the role of arginase II in hypoxic (1% O2, 5% CO2) cellular proliferation. RESULTS: CONCLUSIONS:
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Authors | Bhuvana A Setty, Yi Jin, Peter J Houghton, Nicholas D Yeager, Thomas G Gross, Leif D Nelin |
Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
(Cell Physiol Biochem)
Vol. 39
Issue 2
Pg. 802-13
( 2016)
ISSN: 1421-9778 [Electronic] Germany |
PMID | 27475813
(Publication Type: Journal Article)
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Copyright | © 2016 The Author(s) Published by S. Karger AG, Basel. |
Chemical References |
- Enzyme Inhibitors
- ARG2 protein, human
- Arginase
- Eflornithine
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Topics |
- Arginase
(antagonists & inhibitors, genetics, metabolism)
- Blotting, Western
- Cell Hypoxia
- Cell Line, Tumor
- Cell Proliferation
(drug effects, genetics)
- Cell Survival
(drug effects, genetics)
- Eflornithine
(pharmacology)
- Enzyme Inhibitors
(pharmacology)
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Neoplastic
- Humans
- Osteosarcoma
(enzymology, genetics, pathology)
- RNA Interference
- Reverse Transcriptase Polymerase Chain Reaction
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