Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate.

Abstract

Oviductus Ranae is the dry oviducts of Rana temporaria chensinensis, and it has been reported to have a range of biological activities. This study aimed to investigate the effects of Oviductus Ranae protein hydrolysate (ORPH) on human glioma C6 cell proliferation and apoptosis in vitro and in vivo. Following in vitro treatment, cell viability and colony formation assays showed that ORPH inhibited C6 cell proliferation. In addition, the results of western blotting also demonstrated that ORPH effectively regulated the expression of the apoptosis related proteins, cleaved caspase-3, Bax and Bcl-2, DNA staining and flow cytometry analysis demonstrated that ORPH significantly promoted apoptosis in this cell line, a finding that was confirmed in vivo using terminal deoxynucleotidyl transferase dUTP nick end labeling. Further investigation demonstrated that ORPH increased apoptosis by modulating the release of inflammatory cytokines and the phosphoinositide 3-kinase (PI3K)/AKT signaling pathway; this was demonstrated using a PI3K/AKT inhibitor (NVP-BEZ235). In summary, the present study suggested that ORPH promoted apoptosis and inhibited glioma cell proliferation by influencing the PI3K/AKT signaling pathway.

• Authors: Xin Sui, Xiao-Hua Li, Ming-Hua Duan, Ai-Ling Jia, Ye Wang, Da Liu, Yi-Ping Li, Zhi-Dong Qiu
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 81 Pg. 176-181 (Jul 2016) ISSN: 1950-6007 [Electronic] France
• PMID: 27261592 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Chemical References

• Cytokines
• Materia Medica
• Oviductus Ranae
• Protein Hydrolysates
• Phosphatidylinositol 3-Kinases
• Proto-Oncogene Proteins c-akt

Topics

• Animals
• Apoptosis (drug effects)
• Brain Neoplasms (enzymology, pathology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cytokines (metabolism)
• Enzyme Activation (drug effects)
• Glioma (enzymology, pathology)
• Humans
• Inflammation (pathology)
• Materia Medica (pharmacology)
• Mice, Inbred BALB C
• Mice, Nude
• Phosphatidylinositol 3-Kinases (metabolism)
• Protein Hydrolysates (pharmacology)
• Proto-Oncogene Proteins c-akt (metabolism)
• Signal Transduction (drug effects)