Role of alkaline phosphatase intestine-isomerase in acute mesenteric ischemia diagnosis.

Abstract

BACKGROUND:

The aim of the present study was to investigate the diagnostic value of alkaline phosphatase (ALP) intestine-isomerase, plasma lactate dehydrogenase (LDH), and D-dimer levels in acute mesenteric ischemia.

METHODS:

Thirty Wistar rats were divided into 5 groups of 6 rats each. In Group 1, blood samples were obtained to determine normal parameter levels. In the sham group, Group 2, blood samples were obtained following laparotomy. In Group 3, blood samples were obtained 2 hours after ligation. In Groups 4 and 5, blood samples were obtained at 4 and 6 hours after ligation, respectively. Ischemic damage was assessed using a pathological scoring system. Blood samples were analyzed for hourly changes in parameters.

RESULTS:

No statistically significant difference in D-dimer levels was found between ischemia groups (p=0.337). A statistically significant difference in LDH levels was found between the control group, Group 1, and Group 4 (p=0.018). ALP intestine-isomerase enzyme levels were not statistically significant in other groups (p=0.077).

CONCLUSION:

Findings indicate that plasma LDH levels higher than 1900 IU/L may be a useful marker in the early diagnosis of acute mesenteric obstruction. However, ALP intestine-isomerase enzyme and D-dimer plasma levels were not found to contribute to the diagnosis.

• Authors: Emin Lapsekili, Öner Menteş, Müjdat Balkan, Armağan Günal, Halil Yaman, Orhan Kozak, Yusuf Peker
• Journal: Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES (Ulus Travma Acil Cerrahi Derg) Vol. 22 Issue 2 Pg. 115-20 (Mar 2016) ISSN: 1307-7945 [Electronic] Turkey
• PMID: 27193976 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Fibrin Fibrinogen Degradation Products
• Isoenzymes
• fibrin fragment D
• Alpi protein, rat
• Alkaline Phosphatase
• Isomerases

Topics

• Alkaline Phosphatase (blood)
• Animals
• Biomarkers (blood)
• Female
• Fibrin Fibrinogen Degradation Products (metabolism)
• Isoenzymes (blood)
• Isomerases (blood)
• Mesenteric Ischemia (blood, diagnosis)
• ROC Curve
• Rats
• Rats, Wistar
• Sensitivity and Specificity