Effects of metformin and sitagliptin on glycolipid metabolism in type 2 diabetic rats on different diets.

Abstract

INTRODUCTION:

The aim of the study was to investigate the effects of metformin and sitagliptin on glycolipid metabolism in type 2 diabetes after different diets.

MATERIAL AND METHODS:

Seventy Male Sprague Dawley rats were fed with a high fat diet followed by streptozotocin treatment to induce type 2 diabetes. Then all rats were randomly divided into a control group, a metformin group (200 mg/kg), and a sitagliptin group (10 mg/kg). Each group was further divided into 4 groups receiving one load of high carbohydrate diet (45% glucose, 4.5 ml/kg), high fat diet (20% lipid emulsion, 4.5 ml/kg), high protein diet (20% whey protein, 10 ml/kg) or mixed meal, respectively. The caloric densities were all 33 kJ/kg. Postprandial blood glucose (P2BG), triglyceride (TG), glucagon-like peptide-1 (GLP-1), glucagon and insulin levels were measured.

RESULTS:

In the high carbohydrate group, sitagliptin was more efficient in lowering P2BG compared with metformin (p < 0.05). In the high-fat group, metformin was more powerful in lowering TG (p < 0.05) and P2BG (p < 0.05) levels because of its improvement of insulin sensitivity. In the high protein diet group, metformin did not reduce the P2BG level (p > 0.05), although it did reduce the TG level (p < 0.05). In the mixed diet group, metformin was more efficient in lowering P2BG (p < 0.05) but had a similar effect on TG (p > 0.05) compared with sitagliptin.

CONCLUSIONS:

In the type 2 diabetic model, metformin and sitagliptin have different effects on glycolipid metabolism after different diets. If it is proved in type 2 diabetic patients, then different medicines may be recommended according to different diets in order to improve glycolipid metabolism.

• Authors: Juhong Yang, Tu Ba, Liming Chen, Chunyan Shan, Miaoyan Zheng, Ying Wang, Huizhu Ren, Jingli Chen, Jie Xu, Fei Han, Yi Zhang, Xiaoyun Yang, Baocheng Chang
• Journal: Archives of medical science : AMS (Arch Med Sci) Vol. 12 Issue 2 Pg. 233-42 (Apr 01 2016) ISSN: 1734-1922 [Print] Poland
• PMID: 27186166 (Publication Type: Journal Article)