Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray.

Abstract

Cassava anthracnose disease (CAD), caused by the fungus Colletotrichum gloeosporioides f. sp. Manihotis, is a serious disease of cassava (Manihot esculenta) worldwide. In this study, we established a cassava oligonucleotide-DNA microarray representing 59,079 probes corresponding to approximately 30,000 genes based on original expressed sequence tags and RNA-seq information from cassava, and applied it to investigate the molecular mechanisms of resistance to fungal infection using two cassava cultivars, Huay Bong 60 (HB60, resistant to CAD) and Hanatee (HN, sensitive to CAD). Based on quantitative real-time reverse transcription PCR and expression profiling by the microarray, we showed that the expressions of various plant defense-related genes, such as pathogenesis-related (PR) genes, cell wall-related genes, detoxification enzyme, genes related to the response to bacterium, mitogen-activated protein kinase (MAPK), genes related to salicylic acid, jasmonic acid and ethylene pathways were higher in HB60 compared with HN. Our results indicated that the induction of PR genes in HB60 by fungal infection and the higher expressions of defense response-related genes in HB60 compared with HN are likely responsible for the fungal resistance in HB60. We also showed that the use of our cassava oligo microarray could improve our understanding of cassava molecular mechanisms related to environmental responses and development, and advance the molecular breeding of useful cassava plants.

• Authors: Yoshinori Utsumi, Maho Tanaka, Atsushi Kurotani, Takuhiro Yoshida, Keiichi Mochida, Akihiro Matsui, Manabu Ishitani, Supajit Sraphet, Sukhuman Whankaew, Thipa Asvarak, Jarunya Narangajavana, Kanokporn Triwitayakorn, Tetsuya Sakurai, Motoaki Seki
• Journal: Journal of plant research (J Plant Res) Vol. 129 Issue 4 Pg. 711-726 (Jul 2016) ISSN: 1618-0860 [Electronic] Japan
• PMID: 27138000 (Publication Type: Journal Article)

Chemical References

• Cyclopentanes
• Ethylenes
• Oxylipins
• jasmonic acid
• ethylene
• Salicylic Acid

Topics

• Colletotrichum (physiology)
• Cyclopentanes (metabolism)
• Ethylenes (metabolism)
• Gene Expression Profiling (methods)
• Gene Expression Regulation, Plant
• Gene Ontology
• Genes, Plant
• Manihot (genetics, microbiology)
• Oligonucleotide Array Sequence Analysis (methods)
• Oxylipins (metabolism)
• Plant Diseases (genetics, microbiology)
• Real-Time Polymerase Chain Reaction
• Reproducibility of Results
• Salicylic Acid (metabolism)
• Signal Transduction (genetics)
• Up-Regulation (genetics)