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The effects of NONRATT021972 lncRNA siRNA on PC12 neuronal injury mediated by P2X7 receptor after exposure to oxygen-glucose deprivation.

Abstract
Adenosine triphosphate (ATP) participates in signal transmission by acting on P2X receptors, and the P2X7 receptor is involved in the pathophysiological changes of ischemic injury. The PC12 cell line is a popular model system to study sympathetic neuronal function. Long noncoding RNAs (lncRNAs) are highly expressed in the nervous system and serve as regulatory RNAs. In this study, the effects of NONRATT021972 lncRNA siRNA on P2X7-mediated PC12 neuronal injury after exposure to oxygen-glucose deprivation (OGD) were investigated. Our results showed that the viability of PC12 cells cultured with OGD or the P2X7 agonist BzATP was significantly decreased. Treatment with NONRATT021972 siRNA reversed the decreased viability of PC12 cells under OGD conditions. The upregulated P2X7 mRNA and protein levels in PC12 cells under OGD conditions or BzATP treatment were significantly decreased when pretreated with NONRATT021972 siRNA. Moreover, NONRATT021972 siRNA treatment effectively suppressed the increase in [Ca(2+)]i induced by OGD or P2X7 agonists (ATP or BzATP) in PC12 cells. Therefore, treatment with NONRATT021972 siRNA may decrease sympathetic neuronal injury induced by ischemia.
AuthorsGuilin Li, Lifang Zou, Wei Xie, Shiyao Wen, Qiuyu Xie, Yun Gao, Changshui Xu, Hong Xu, Shuangmei Liu, Shouyu Wang, Yun Xue, Bing Wu, Qiulan Lv, Mofeng Ying, Xi Zhang, Shangdong Liang
JournalPurinergic signalling (Purinergic Signal) Vol. 12 Issue 3 Pg. 479-87 (09 2016) ISSN: 1573-9546 [Electronic] Netherlands
PMID27100355 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • RNA, Long Noncoding
  • Receptors, Purinergic P2X7
  • long non-coding RNA NONRATT021972, rat
  • Glucose
  • Oxygen
Topics
  • Animals
  • Blotting, Western
  • Cell Hypoxia
  • Cell Survival (drug effects)
  • Disease Models, Animal
  • Gene Knockdown Techniques
  • Glucose (deficiency)
  • Ischemia (metabolism)
  • Oxygen
  • PC12 Cells
  • Polymerase Chain Reaction
  • RNA, Long Noncoding (metabolism, pharmacokinetics)
  • Rats
  • Receptors, Purinergic P2X7 (metabolism)

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