Effect of Ligustrazine on rat peritoneal mesothelial cells treated with lipopolysaccharide.

Abstract

The apoptosis of peritoneal mesothelial cells (PMCs) and peritoneal fibrosis may induce failure of peritoneal membrane function. The study explored the changes of apoptosis and fibrosis in PMCs under lipopolysaccharides (LPS) culture and investigated whether Ligustrazine can affect LPS-induced apoptosis and fibrosis. We found that exposure of rat PMCs to 5 mg·L(-1) LPS for 24 h resulted in a significant induction of apoptosis and increased levels in Reactive oxygen species, and caspase-3 activity. Fibronectin, Collagen I, p-p38, and matrix metalloprotein-9 (MMP-9) levels were also significantly increased by LPS. But superoxide dismutase levels were remarkably decreased. Ligustrazine can restore the changes induced by LPS. The protective effect of Ligustrazine on LPS-induced apoptosis and fibrosis may act through inhibition of oxidative stress and p38/MAPKS, ROS/MMP-9 activation in PMCs.

• Authors: Hui Zhang, Dongxia Li, Zhiyong Li, Yu Song
• Journal: Renal failure (Ren Fail) Vol. 38 Issue 6 Pg. 961-9 (Jul 2016) ISSN: 1525-6049 [Electronic] England
• PMID: 27056404 (Publication Type: Journal Article)

Chemical References

• Collagen Type I
• Fibronectins
• Lipopolysaccharides
• Pyrazines
• Reactive Oxygen Species
• p38 Mitogen-Activated Protein Kinases
• Casp3 protein, rat
• Caspase 3
• Matrix Metalloproteinase 9
• Mmp9 protein, rat
• tetramethylpyrazine

Topics

• Animals
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cells, Cultured
• Collagen Type I (metabolism)
• Epithelial Cells (drug effects)
• Fibronectins (metabolism)
• Lipopolysaccharides (pharmacology)
• Male
• Matrix Metalloproteinase 9 (metabolism)
• Oxidative Stress (drug effects)
• Peritoneal Fibrosis (metabolism)
• Pyrazines (pharmacology)
• Rats
• Rats, Wistar
• Reactive Oxygen Species (metabolism)
• p38 Mitogen-Activated Protein Kinases (metabolism)