Characterization of surface antigen protein 1 (SurA1) from Acinetobacter baumannii and its role in virulence and fitness.

Abstract

Acinetobacter baumannii is a Gram-negative bacillus that causes nosocomial infections, such as bacteremia, pneumonia, and meningitis and urinary tract and wound infections. In the present study, the surface antigen protein 1 (SurA1) gene of A. baumannii strain CCGGD201101 was identified, cloned and expressed, and then its roles in fitness and virulence were investigated. Virulence was observed in the human lung cancer cell lines A549 and HEp-2 at one week after treatment with recombinant SurA1. One isogenic SurA1 knock-out strain, GR0015, which was derived from the A. baumannii strain CCGGD201101 isolated from diseased chicks in a previous study, highlighted the effect of SurA1 on fitness and growth. Its growth rate in LB broth and killing activity in human sera were significantly decreased compared with strain CCGGD201101. In the Galleria mellonella insect model, the isogenic SurA1 knock-out strain exhibited a lower survival rate and decreased dissemination. These results suggest that SurA1 plays an important role in the fitness and virulence of A. baumannii.

• Authors: Dong Liu, Zeng-Shan Liu, Pan Hu, Ling Cai, Bao-Quan Fu, Yan-Song Li, Shi-Ying Lu, Nan-Nan Liu, Xiao-Long Ma, Dan Chi, Jiang Chang, Yi-Ming Shui, Zhao-Hui Li, Waqas Ahmad, Yu Zhou, Hong-Lin Ren
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 186 Pg. 126-38 (Apr 15 2016) ISSN: 1873-2542 [Electronic] Netherlands
• PMID: 27016767 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies, Bacterial
• Antigens, Surface
• Recombinant Proteins

Topics

• Acinetobacter Infections (microbiology)
• Acinetobacter baumannii (genetics, growth & development, pathogenicity, physiology)
• Amino Acid Sequence
• Animals
• Antibodies, Bacterial (immunology)
• Antigens, Surface (genetics, metabolism)
• Base Sequence
• Biofilms
• Cell Line, Tumor
• Cell Survival (drug effects)
• Chickens
• Gene Expression Regulation
• Gene Knockout Techniques
• Humans
• Molecular Sequence Data
• Moths (microbiology)
• Plasmids (genetics)
• Recombinant Proteins (genetics, toxicity)
• Virulence (genetics)